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Venom peptidomics and transcriptomics of the spider Nephilengys cruentata

Grant number: 14/17140-2
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2014
Effective date (End): November 30, 2016
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Alexandre Keiji Tashima
Grantee:Rafael Lucena Lomazi
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The diversity of biological activities found spider venom components has motivated many research groups to search for molecules with potential pharmacological use, which results in an increasing number of toxins isolated and sequenced in recent years. But despite the advances, the number of peptides isolated and characterized spiders is estimated to be 0.01% of all possible existing sequences. Studies indicated the presence of peptides with antimicrobial activity in the venom of several Brazilian spiders, however, few of these components have been characterized so far. Considering the biological potential of the molecules contained in the venoms of Nephilengys cruentata spider, the importance of characterizing these toxins and the importance of exploring new molecules with antimicrobial activity, we propose in this master's project the fractionation of peptides present in the venom of the spider Nephilengys cruentata, the identification of molecules with antimicrobial activity and the determination of their structures by mass spectrometry analysis and search in a transcriptomic database from venom glands. (AU)