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Role of the small RNA CCNA_R0074 in the regulation of translesion synthesis in Caulobacter crescentus

Grant number: 14/24571-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2015
Effective date (End): February 29, 2016
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Rodrigo da Silva Galhardo
Grantee:Ellen Kazumi Okuda
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Caulobacter crescentus is a free living bacterium, which presents different cellular forms that allow cell cycle studies, making it a widely used model. Recently, 27 new small RNAs (sRNAs) were identified in the C. crescentus genome. Little is known about the mechanism of action of sRNAs, which can target mRNAs, proteins or both. When mRNAs are the target, sRNAs act by base pairing with them, in such a way that they may either stimulate or prevent translation, by promoting RNA stability or by inhibition of translation start, respectively. Among the sRNAs present in this bacterium, one is present between the imuA and imuB genes, which together with dnaE2, belong to an SOS-regulated operon involved in translesion synthesis. In this project, we will investigate if this sRNA is involved in the regulation of the imuABdnaE2 genes.