Prostate tissue response after treatment with Nintedanibe in transgenic mice for adenocarcinoma (TRAMP) at different stages of tumor development

• Grant number: 13/26677-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2015
• End date: March 01, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Valéria Helena Alves Cagnon Quitete
• Grantee: Raquel Frenedoso da Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/13913-2 - Molecular mechanism based of Nintedanib on prostate cancer therapy, BE.EP.DR

Abstract

Prostate cancer has been widely studied due to the large number of individuals affected by this type of injury, in addition to the complexity of the operation and development of this organ considering the important epithelial-stromal interaction as a primary factor for prostate alteration. Special attention has been paid to the study of inhibitors of the FGF and VEGF, as Nintedanib (BIBF 1120), a compound capable to inhibit angiogenesis, prevent cell proliferation and decrease tumor volume, used in the prevention, intervention and regression prostate cancer. Since there is no either direct cure by chemical agents for prostate cancer at an advanced stage or effective therapeutic strategies for treatment, it is necessary to priorize new studies with animals models that mimic the disease in man to know effective strategies for prevention and progression of the cancer. In the present study, the transgenic adenocarcinoma of mouse prostate (TRAMP) will be treated with Nintedanibe in 10mg/kg/dia at different stages of tumor development, ie, prostatic malignancy in early stages (8 weeks), intermediate (12 weeks) and advanced (22 weeks). After the end of treatment, the prostate ventral lobe will be collected for analysis of the structural profile, immunohistochemical and Western blotting for protein quantification and toxicity of the drug, analyzing the liver morphology. Furthermore, we will analyze the possible metastatic process and possivle adverse effects of Nintedanib in the testis of treated animals by analysis of sperm quality and quantity and immunohistochemistry. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PANGRAZI, ELLEN NOGUEIRA; DA SILVA, RAQUEL F.; KIDO, LARISSA A.; MONTICO, FABIO; CAGNON, VALERIA H. A.. Nintedanib treatment delays prostate dorsolateral lobe cancer progression in the TRAMP model: contribution to the epithelial-stromal interaction balance. Cell Biology International, v. 42, n. 2, p. 153-168, FEB 2018. (13/26677-7)

DA SILVA, RAQUEL FRENEDOSO; BANZATO, THAIS PETROCHELLI; ALVES, LETICIA FERREIRA; CARVALHO, JOAO ERNESTO; AGARWAL, RAJESH; CAGNON, VALERIA HELENA ALVES. Antiangiogenic therapy with Nintedanib affects hypoxia, angiogenesis and apoptosis in the ventral prostate of TRAMP animals. Cell and Tissue Research, v. 379, n. 2, p. 407-420, FEB 2020. (13/26677-7)

DA SILVA, RAQUEL FRENEDOSO; RAINA, KOMAL; KUMAR, DILEEP; DHAR, DEEPANSHI; ALVES CAGNON, VALERIA HELENA; AGARWAL, CHAPLA; AGARWAL, RAJESH. Antiangiogenic Agent Nintedanib (BIBF 1120) Effectiveness in vitro and in vivo Against Prostate Cancer. FASEB JOURNAL, v. 31, p. 1-pg., 2017-04-01. (13/26677-7)

DA SILVA, RAQUEL FRENEDOSO; NOGUEIRA-PANGRAZI, ELLEN; KIDO, LARISSA AKEMI; MONTICO, FABIO; ARANA, SARAH; KUMAR, DILEEP; RAINA, KOMAL; AGARWAL, RAJESH; ALVES CAGNON, VALERIA HELENA. Nintedanib antiangiogenic inhibitor effectiveness in delaying adenocarcinoma progression in Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP). JOURNAL OF BIOMEDICAL SCIENCE, v. 24, MAY 12 2017. (13/26677-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Raquel Frenedoso da. Antiangiogenic action of Nintedanib in prostate tumor cells and in the prostate of transgenic mice for adenocarcinoma (TRAMP) in different stages of tumor development. 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.