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Regeneration process characteristics of skeletal muscle of different embryological origins in mdx mice submitted to aerobic exercise

Grant number: 14/22211-6
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2015
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Flávia de Oliveira
Grantee:Mariana Cruz Lazzarin
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil


Duchenne Muscular Dystrophy (DMD) is a genetic disease that causes loss of muscle functionality, strength and mass. The effects of exercise on dystrophic muscle are not well established. The purpose of this study is to analyze the effects of aerobic exercise training on the morphology and on myogenic markers of muscle regeneration and inflammation as well as oxidative stress in skeletal muscle of different embryological origins in mdx mice. C57BL10 mice (n=14) and C57BL10-DMD/mdx (n=14), with eight weeks old will be equally distributed in four groups: sedentary control, trained control, sedentary mdx and trained mdx. All animals from trained groups will be submitted to aerobic exercise training, one hour a day (17 m/min), 5 days a week for 7 weeks. After euthanasia, the gastrocnemius muscles (myotonic origin) and masseter (branchiomeric origin) will be dissected and processed for: histopathology analysis; immunohistochemical analysis - muscle regeneration (MyoD and Myogenin), inflammation (COX-2) and oxidative stress (8-OHdG); and morphometric analysis (fibers cross section area and cell density). The hypothesis of the present study is based on the exercise can be change the histopathological, morphometric and inflammatory parameters in different ways to gastrocnemius and masseter muscles. The results of present study can contribute to knowledge and treatment of DMD. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA, LIDIANE BEGALLI; MAZIERO, CARLA; LAZZARIN, MARIANA CRUZ; QUINTANA, HANANIAH TARDIVO; TOME, TABATA DE CARVALHO; DE ARAUJO BAPTISTA, VIVIANNE IZABELLE; DE OLIVEIRA, FLAVIA. Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice. ACTA HISTOCHEMICA, v. 122, n. 1 JAN 2020. Web of Science Citations: 0.
MARIANA CRUZ LAZZARIN; HANANIAH TARDIVO QUINTANA; VIVIANNE IZABELLE DE ARAÚJO BAPTISTA; FLAVIA DE OLIVEIRA. Lack of dystrophin influences muscle inflammation but not myogenic regulatory factors after eccentric exercise in mdx mice. Motriz: rev. educ. fis., v. 26, n. 3, p. -, 2020.

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