Incidence of 4-nitroquinoline 1-oxide (4NQO)- induced oral squamous cell carcinoma in rats with sympathetic nervous system hyperactivity

• Grant number: 14/25393-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2015
• Effective date (End): April 30, 2018
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Daniel Galera Bernabé
• Grantee: Felipe Yudi Kabeya Lopes
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Recent evidences show that sympathetic nervous system (SNS) hyperactivation induced by chronic stress can influence cancer evolution. In the present study we will use Spontaneous Hypertensive Rats (SHR), a strain of rats with sympathetic nervous system hyperactivity to analyze the effects of sympathetic hiperactivity on the incidence of 4NQO-induced oral squamous cell carcinoma (OSCC). 30 male wistar rats and 30 male SHR rats will be submitted to oral carcinogenesis with 4NQO for 5 months when they will be euthanized to assess the incidence and tongue SCC invasion. To analyze the effects of the sympathetic hiperactivity on the progression tumor-related genes, IL-6, MMP-2, MMP-9, VEGF and p16 expression in tumors will be evaluated by real time PCR. (AU)