Application of NMR-Based metabolomics to biomedical problems

• Grant number: 15/02534-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 01, 2015
• End date: June 30, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Luiz Alberto Colnago
• Grantee: Paulo Falco Cobra
• Supervisor: John Lute Markley
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Institution abroad: University of Wisconsin-Madison (UW-Madison), United States
• Associated to the scholarship: 12/12847-5 - Analysis of leishmaniasis metabolic profile by NMR, BP.DR

Abstract

Nuclear Magnetic Resonance (NMR) nowadays is an important tool in the study of metabolites and has been used for over 20 years to determine metabolic profiles. Metabolic research is usually divided into metabolomics (the identification and quantification of biological system or organism's metabolites, kept under normal conditions) and metabonomics (the analysis of these metabolites when these biological systems or organisms undergo changes in response to pathophysiological stimuli or genetic modification). A great advantage of the technique is that sample preparation for NMR metabolomics experiments is relatively minimal. Using a simple technique, it is possible to identify 20 to 40 metabolites in tissue extracts, 30 to 100 metabolites in urine and up to 65 metabolites in blood plasma or serum. For metabolite identification in NMR spectra, ChenoMX® software is used. However, this software often provides numerous hits on unknown metabolites, and, hence, can lead to incorrect metabolite identification. Thereby, the main goal of this internship project is to learn the metabolite identification methodologies already in use by the NMRFAM group and participate in the developing and testing of the ones that are still under development. The methodology used by the group will allow better and more accurate identification of Leishmania sp. metabolites, the student's PhD topic. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GANDHI, N. N.; COBRA, P. F.; STEELE, J. L.; MARKLEY, J. L.; RANKIN, S. A.. Lactobacillus demonstrate thiol-independent metabolism of methylglyoxal: Implications toward browning prevention in Parmesan cheese. JOURNAL OF DAIRY SCIENCE, v. 101, n. 2, p. 968-978, FEB 2018. (12/12847-5, 15/02534-8)