Verification of value isotopic cheese produced with milk cows fed with poultry litter

• Grant number: 14/23886-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2015
• End date: July 31, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Cyntia Ludovico Martins
• Grantee: Antonio Marcos Silvestre
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Consumers are increasingly concerned about the safety of food, forcing countries to deploy new barriers and laws. The need for methods to detect the end product, which was supplied to the animal, one meets the demands of the consumer market to characterize the shape which the product was conducted prior to its manufacture. The use of products intended for feeding ruminants and containing in their protein composition and fat of animal origin is prohibited. Several studies have highlighted that the use of the technique of stable isotopes is possible to track the type of feed given to the animal through the analysis of these products. In this context, the aim of this work is to identify the isotopic value of carbon and nitrogen in cheese produced from dairy cows fed poultry litter. The trial period is 90 days, where 12 crossbred cows milk will be divided into two treatments, treatment with control diet strictly vegetable and the second with the addition of manure in the diet. 12 liters of milk per cow will be collected on days 56 and 90, for making the type unripened cheeses, totaling 24 cheeses. The cheese samples are ground in a cryogenic mill and then frozen at -20 ° C until further analysis. Isotopic analyzes of ´13C and ´15N will be held at the Center for Stable Isotope Institute of Biosciences, UNESP, Botucatu/SP. The isotopic data will be analyzed jointly for each treatment evaluated by multivariate principal component analysis in Minitab 16 program.