Permeability studies of insulin using Caco-2 cell monolayer and effect of enzyme inhibitory from gellan microcapsules coated with resistant starch/pectin films

• Grant number: 15/01268-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): May 25, 2015
• Effective date (End): August 24, 2015
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Beatriz Stringhetti Ferreira Cury
• Grantee: Andréia Bagliotti Meneguin
• Supervisor: Eric Beyssac
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Research place: Université d'Auvergne (UdA), France
• Associated to the scholarship: 12/11798-0 - Gellan gum microcapsules coated with resistant starch/pectin as a strategy for oral administration of insulin., BP.DR

Abstract

Diabetes mellitus (DM), a chronic metabolic disease in which blood glucose levels are increased, is the sixth most common cause of death worldwide. Its treatment involves the use of oral hypoglycemic drugs and, in severe cases, daily subcutaneous administration of insulin (INS), as it is an invasive route often leads to non-adherence of the patient to the treatment. In this sense, different strategies have been evaluated in order to find an alternative to INS administration, and the oral route is considered most convenient, safe and natural. However, oral administration of protein drugs is a major problem, mainly due to enzymatic degradation suffered in the upper portions of the gastrointestinal tract and low permeability across the intestinal mucosa. Thus, the encapsulation of INS in gellan microcapsules coated with resistant starch/pectin should promote the INS protection against degradation in the upper portions of the GI tract, allowing the release only the end portion of the bowel (colon specific release) and providing a contact closer to the intestinal mucosa, which should favor the permeation. During the research stage at the Faculty of Pharmacy (University D'Avergne) in Clermont-Ferrand, France, under the supervision of Prof. Dr. Eric Beyssac, gellan microcapsules coated with resistant starch / pectin will be assessed for its protective effect against the enzymatic degradation of INS and for its impact on increasing the bioavailability through permeability studies in Caco-2 cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENEGUIN, ANDREIA B.; BEYSSAC, ERIC; GARRAIT, GHISLAIN; HSEIN, HASSANA; CURY, BEATRIZ S. F.. Retrograded starch/pectin coated gellan gum-microparticles for oral administration of insulin: A technological platform for protection against enzymatic degradation and improvement of intestinal permeability. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 123, p. 84-94, FEB 2018. (15/01268-2)