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Sensitivity study to oxygen reactive species during sperm maturation in dogs

Grant number: 15/00597-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2015
Effective date (End): December 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Cristina de Fátima Lúcio
Grantee:Roberto Rodrigues da Rosa Filho
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Currently, several studies have focused on understanding the physiology of sperm maturation in dogs, however, the lack of susceptibility of sperm epididymal the action of free radicals preclude the use of antioxidants in extender for cryopreservation of such samples. The aim of this study was to evaluate the susceptibility of canine epididymal sperm from head, body and tail to reactive oxygen species. Ten male dogs will undergo orchiectomy. After castration the epididymis will be stored at 5 ° C and the semen will be recovered within 24 hours. The samples will be obtained from the 3 regions of the epididymis: head, body and tail. The samples will be divided and incubated at 37°C for 30 minutes in 5 treatments: superoxide anion (O2-), hydrogen peroxide H2O2, hydroxyl radical (OH-), malondialdehyde and control. After incubation, the samples shall be subjected to computer analysis of sperm motility (CASA), plasma membrane integrity (eosin/nigrosine), acrosome integrity (Fast green/Rose Bengal), mitochondrial activity by oxidation of 3,3 'diaminobenzidine (DAB) and oxidative stress analysis by evaluation of thiobarbituric acid reactive species. The results will be evaluated by the SAS System for Windows (SAS, 2000) considering p <0.05.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA ROSA FILHO, ROBERTO R.; ANGRIMANI, DANIEL S. R.; BRITO, MAIRA M.; NICHI, MARCILIO; VANNUCCHI, I, CAMILA; LUCIO, CRISTINA F.. Susceptibility of epididymal sperm against reactive oxygen species in dogs. ANIMAL BIOTECHNOLOGY, v. 32, n. 1, . (15/00597-2)

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