Isolation and evaluation of commensal lactic acid bacteria effectiveness on inflammatory and microbiological parameters associated with ulcerative colitis

• Grant number: 14/15130-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2015
• End date: October 31, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Daniela Cardoso Umbelino Cavallini
• Grantee: Larissa Sbaglia Celiberto
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 15/21958-3 - Effect of probiotic consumption on the fecal microbiota in the dextran sodium sulphate colitis mouse model, BE.EP.DR

Abstract

The use of certain lactic acid bacteria has been considered as a possible and promising therapeutic approach on reducing the risk of inflammatory bowel disease (IBD), more precisely ulcerative colitis (UC). This work aims to study the effectiveness of daily intake of commensal lactic acid bacteria strains isolated from animal faeces, with probiotic potential effect, in different stages of chemically induced colitis in mice in order to investigate the possible mechanisms associated. Colitis will be induced by 3% dextran sulfate sodium (DSS), being studied two phases of colitis: acute (14 days) and chronic (28 days). The animals will then be randomly assigned into four groups (n=10): Group C: healthy animals that do not receive the microorganisms under study; Group CL: animals with colitis that do not receive the microorganisms under study; Group CLC: animals with colitis that receive a mixture of probiotics strains (E. faecium CRL 183, L. helveticus 416 and B. longum ATCC 15707); Group CLP: animals with colitis that receive the strains isolated from their own faeces (individualized treatment). The strains of lactic acid bacteria isolated from animal feces will be submitted to analysis of gastrointestinal environment resistance, antibiotic sensitivity and specie/strain identification by molecular techniques. Throughout the experimental protocol the following parameters will be monitored: disease activity index, short-chain fatty acids concentration in the faeces, and intestinal microbiota composition by independent growth techniques. At the end of each stage of colitis, the animals will be euthanized and the large intestine will be removed to perform histological and immunohistochemistry analysis in the colon, to determine the concentration of cytokines (IL-1², IL-6, IL-10, IL12, TGF- ² e TNF-±), biochemistry markers (MPO activity, MDA and NO) and protein expression (ZO1, claudin 1, 2 e 3 and occludin). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CELIBERTO, LARISSA S.; PINTO, ROSELI APARECIDA; ROSSI, ELIZEU ANTONIO; VALLANCE, BRUCE A.; CAVALLINI, DANIELA C. U.. Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics. NUTRIENTS, v. 10, n. 11, NOV 2018. (14/15129-1, 14/15130-0, 15/21958-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CELIBERTO, Larissa Sbaglia. Intestinal homeostasis and host defense as promoted by commensal bacteria and the colonic mucus layer. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.