| Grant number: | 14/26131-7 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | May 01, 2015 |
| End date: | April 30, 2019 |
| Field of knowledge: | Biological Sciences - Microbiology - Biology and Physiology of Microorganisms |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Itamar Soares de Melo |
| Grantee: | Diego Bonaldo Genuário |
| Host Institution: | Embrapa Meio-Ambiente. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Jaguariúna , SP, Brazil |
| Associated scholarship(s): | 17/15190-0 - Heterologous Expression of Selected Gene Clusters Identified in Isolated Amazonian Cyanobacterial Strain, BE.EP.PD |
Abstract The Amazon River basin is the largest river basin in the world, comprising about 40% of the total area of the South America. The Amazon River is the largest river in the world in terms of volumetric discharge, and possesses a length of 6.280 km, a catchment area of about 7.000 km2 and runoff amounting to nearly 15% of the total runoff of all the rivers in the world together. The fresh water discharged by this river is transported hundreds to thousands of kilometers away from the coast by surface plumes. As consequence, the Amazon River delivers great amounts nutrients into the ocean such as iron, phosphorous and silicon enhancing the primary production in the ocean and the carbon sequestration by its sinking. However, the primary production does not suffer limitation due to the nitrogen availability supplied by the biological nitrogen fixation mediate mainly by cyanobacteria. Thus, the aims of this study are characterize and quantify the diazotrophic community present in the Amazon River and its tributaries (Rio Negro and Solimões) with special emphasis on cyanobacterial communities. This effort will provides a first glimpse in the functional characteristics of the microbiota of the Amazon River and its main tributaries focusing on diazotrophic communities and biological nitrogen fixation.The investigation of bacterial and diazotrophic communities present in water samples will be carried out through the massive sequencing, whereas the abundance of these communities will be held by quantitative PCR.In both approaches the target genes coding for 16S rRNA and nifH be investigated from metagenomic DNA. Yet, estimation of nitrogenase activity will be investigated by the acetylene reduction assay in environmental water samples and isolation of cyanobacterial strains will be made using specific culture media for this group of microorganisms. (AU) | |
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