Microbiome of Amazon River and its tributaries: biogeography and contribution to N2 cycle

• Grant number: 14/26131-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): May 01, 2015
• Effective date (End): April 30, 2019
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Itamar Soares de Melo
• Grantee: Diego Bonaldo Genuário
• Host Institution: Embrapa Meio-Ambiente. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). Jaguariúna , SP, Brazil
• Associated scholarship(s): 17/15190-0 - Heterologous Expression of Selected Gene Clusters Identified in Isolated Amazonian Cyanobacterial Strain, BE.EP.PD

Abstract

The Amazon River basin is the largest river basin in the world, comprising about 40% of the total area of the South America. The Amazon River is the largest river in the world in terms of volumetric discharge, and possesses a length of 6.280 km, a catchment area of about 7.000 km2 and runoff amounting to nearly 15% of the total runoff of all the rivers in the world together. The fresh water discharged by this river is transported hundreds to thousands of kilometers away from the coast by surface plumes. As consequence, the Amazon River delivers great amounts nutrients into the ocean such as iron, phosphorous and silicon enhancing the primary production in the ocean and the carbon sequestration by its sinking. However, the primary production does not suffer limitation due to the nitrogen availability supplied by the biological nitrogen fixation mediate mainly by cyanobacteria. Thus, the aims of this study are characterize and quantify the diazotrophic community present in the Amazon River and its tributaries (Rio Negro and Solimões) with special emphasis on cyanobacterial communities. This effort will provides a first glimpse in the functional characteristics of the microbiota of the Amazon River and its main tributaries focusing on diazotrophic communities and biological nitrogen fixation.The investigation of bacterial and diazotrophic communities present in water samples will be carried out through the massive sequencing, whereas the abundance of these communities will be held by quantitative PCR.In both approaches the target genes coding for 16S rRNA and nifH be investigated from metagenomic DNA. Yet, estimation of nitrogenase activity will be investigated by the acetylene reduction assay in environmental water samples and isolation of cyanobacterial strains will be made using specific culture media for this group of microorganisms. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GENUARIO, DIEGO BONALDO; MARCAL VIEIRA VAZ, MARCELO GOMES; DE VIELO, ITAMAR SOARES. Phylogenetic insights into the diversity of homocytous cyanobacteria from Amazonian rivers. Molecular Phylogenetics and Evolution, v. 116, p. 120-135, NOV 2017. (14/26131-7, 13/26659-9)

SOUZA, DANILO T.; GENUARIO, DIEGO B.; SILVA, FABIO SERGIO P.; PANSA, CAMILA C.; KAVAMURA, VANESSA N.; MORAES, FERNANDO C.; TAKETANI, RODRIGO G.; MELO, ITAMAR S.. Analysis of bacterial composition in marine sponges reveals the influence of host phylogeny and environment. FEMS MICROBIOLOGY ECOLOGY, v. 93, n. 1, JAN 2016. (13/03158-4, 14/26131-7, 13/08144-1, 13/25505-8)

GENUARIO, DIEGO BONALDO; DINI ANDREOTE, ANA PAULA; MARCAL VIEIRA VAZ, MARCELO GOMES; FIORE, MARLI FATIMA. Heterocyte-forming cyanobacteria from Brazilian saline-alkaline lakes. Molecular Phylogenetics and Evolution, v. 109, p. 105-112, APR 2017. (10/00321-3, 13/50425-8, 10/18732-0, 14/26131-7, 09/15402-1)

DA SILVA MALONE, CAMILA FRANCIELI; GENUARIO, DIEGO BONALDO; MARCAL VIEIRA VAZ, MARCELO GOMES; FIORE, MARLI FATIMA; SANT'ANNA, CELIA LEITE. Monilinema gen. nov., a homocytous genus (Cyanobacteria, Leptolyngbyaceae) from saline-alkaline lakes of Pantanal wetlands, Brazil(1). JOURNAL OF PHYCOLOGY, v. 57, n. 2, DEC 2020. (10/18732-0, 10/00321-3, 12/18528-9, 14/26131-7)

GERALDES, VANESSA; JACINAVICIUS, FERNANDA RIOS; GENUARIO, DIEGO BONALDO; PINTO, ERNANI. Identification and distribution of mycosporine-like amino acids in Brazilian cyanobacteria using ultrahigh-performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, FEB 2020. (14/50420-9, 14/26131-7)

GENUARIO, DIEGO BONALDO; SANT'ANNA, CELIA LEITE; MELO, ITAMAR SOARES. Elucidating the Cronbergia (cyanobacteria) dilemma with the description of Cronbergia amazonensis sp nov isolated from Solimoes river (Amazonia, Brazil). ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 29, p. 233-241, JAN 2018. (14/26131-7, 13/26659-9)

GENUARIO, DIEGO BONALDO; LORENZI, ADRIANA STURION; AGUJARO, LIVIA FERNANDA; ISAAC, RICARDO DE LIMA; DE PAIVA AZEVEDO, MARIA TERESA; NETO, ROMEU CANTUSIO; FIORE, MARLI FATIMA. Cyanobacterial community and microcystin production in a recreational reservoir with constant Microcystis blooms. Hydrobiologia, v. 779, n. 1, p. 105-125, OCT 2016. (10/00321-3, 14/26131-7)

GENUARIO, DIEGO BONALDO; DE SOUZA, WALLACE RAFAEL; ROSIN MONTEIRO, REGINA TERESA; SANT'ANNA, CELIA LEITE; MELO, ITAMAR SOARES. Amazoninema gen. nov., (Synechococcales, Pseudanabaenaceae) a novel cyanobacteria genus from Brazilian Amazonian rivers. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 68, n. 7, p. 2249-2257, JUL 2018. (14/26131-7, 13/26659-9)

SILVA, LEONARDO JOSE; SOUZA, DANILO TOSTA; GENUARIO, DIEGO BONALDO; VARGAS HOYOS, HAROLD ALEXANDER; SANTOS, SUIKINAI NOBRE; ROSA, LUIZ HENRIQUE; ZUCCHI, TIAGO DOMINGUES; MELO, ITAMAR SOARES. Rhodococcus psychrotolerans sp nov., isolated from rhizosphere of Deschampsia antarctica. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 111, n. 4, p. 629-636, APR 2018. (13/25505-8, 14/26131-7)

VARGAS HOYOS, HAROLD ALEXANDER; SANTOS, SUIKINAI NOBRE; DA SILVA, LEONARDO JOSE; PAULINO SILVA, FABIO SERGIO; GENUARIO, DIEGO BONADO; ZUCCHI, TIAGO DOMINGUES; MELO, ITAMAR SOARES. Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 69, n. 8, p. 2431-2439, AUG 2019. (13/16037-0, 13/25076-0, 14/26131-7)

DE ADVARENGA, LUNA VIGGIANO; MARCAL VIEIRA VAZ, MARCELO GOMES; GENUARIO, DIEGO BONACLO; ESTEVES-FERREIRA, ALBERTO A.; MARTINS ALMEIDA, V, ALLAN; DE CASTRO, NAIRA VALLE; LIZIERI, CLAUDINEIA; SOUZA, JOSE JOAO L. L.; SCHAEFER, CARLOS ERNESTO G. R.; NUNES-NESI, ADRIANO; et al. Extending the ecological distribution of Desmonostoc genus: proposal of Desmonostoc salinum sp nov., a novel Cyanobacteria from a saline-alkaline lake. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 68, n. 9, p. 2770-2782, SEP 2018. (14/26131-7)

HENTSCHKE, GUILHERME S.; RIGONATO, JANAINA; GENUARIO, DIEGO B.; LAUGHINGHOUSE, HAYWOOD D.; SANT'ANNA, CELIA L.. Morphological and molecular characterization of Stigonema jureiensis sp. nov. (Nostocales, Cyanobacteria) from the Atlantic Rainforest, Sao Paulo, Brazil. Fottea, v. 19, n. 2, p. 185-191, 2019. (14/26131-7)

GERALDES, VANESSA; JACINAVICIUS, FERNANDA RIOS; GENUARIO, DIEGO BONALDO; PINTO, ERNANI. Identification and distribution of mycosporine-like amino acids in Brazilian cyanobacteria using ultrahigh-performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, p. 10-pg., 2020-02-08. (14/50420-9, 14/26131-7)