Assessment and comparison of potential cytotoxicity and genotoxicity of (Z) and (E) forms of methyl-4-(3,5-dimethoxystityl) benzoate: an in vitro study in CHO-K1 cells

• Grant number: 14/23627-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2015
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Raquel Alves dos Santos
• Grantee: Gabriela Carvalho Andrade
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Throughout most of history, Nature, especially plants, has provided a source of medicines for the treatment of a wide spectrum of diseases. This huge diversity of organic compounds represents an important source for searching new therapeutic agents which structural modifications can improve their biological activity. Resveratrol is a stilbene considered one of the promising molecules with biological properties for pharmacological purposes. The chemical structure modification is among the strategies to improve de biological properties of resveratrol and results in compounds such as the (E)-4-(3,5-dimethoxystyryl)benzoate (E-DMSA) and (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzoate (Z-DMSB). Considering that genotoxic analysis is an important step for determination of biological properties of new compounds, the objectives of the present study are to evaluate in vitro the genotoxicity of E-DMSA and Z-DMSB in CHO-K1 cell line using the comet and micronucleus assay after cytototoxicity determination by XTT, clonogenic survival and BrdU assays. (AU)