Effects of nutraceuticals sulforaphane, diallyl disulfide and vitamin D in human cancer cell lines: Evaluation of cytotoxicity, genotoxicity, cell migration, epigenetics changes and gene expression

• Grant number: 17/24576-0
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2018 - March 31, 2021
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Lusânia Maria Greggi Antunes
• Grantee: Lusânia Maria Greggi Antunes
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Alexandre Ferro Aissa

Abstract

The transformation of a normal cell into a cancerous phenotype requires stages of initiation, progression, and promotion by altering specific genes. Although predisposition to cancer cannot be signaled out by a single factor, a group of factors place some individuals at a higher risk of acquiring the disease. As determined by epidemiological studies, the predominant forms of cancer are breast, lung, prostate and liver. Although great advances in cancer treatments such as chemotherapy, surgery, and radiation are currently being achieved, their application is associated with numerous adverse side effects. Many nutraceuticals are currently been investigated for their promising chemoprevention of cancer. Cell culture and in vivo studies suggest that ingestion of these nutraceuticals may inhibit certain types of cancers. Nutraceuticals have been shown to exert anticancer effects, including anthocyanins, lycopene, tea polyphenols, genistein, resveratrol, curcumin, sulforaphane and quercetin. The aim of this study will be on mechanistic pathways that can be regulate in vitro by nutraceuticals sulforaphane, diallyl disulfide and vitamin D in human cancer cell lines (HepG2, PC-3 and DU-145) and non-cancer cells (GAS, HUVEC and PNT2). Various biological endpoints can be measured in in vitro assays, with the nutraceuticals alone or in combination (sulforaphane + vitamin D or diallyl disulfide + sorafenib) with other compounds. Cytotoxicity, cell death by apoptosis and autophagy, migration and cell invasion, genotoxicity and gene expression will be performed. In addition, we will analyze proteins by western blot and identify epigenetic modifications. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA DOS SANTOS, PATRICK WELLINGTON; THOMAZELA MACHADO, ANA RITA; DE GRANDIS, RONE APARECIDO; RIBEIRO, DIEGO LUIS; TUTTIS, KATIUSKA; MORSELLI, MARCO; AISSA, ALEXANDRE FERRO; PELLEGRINI, MATTEO; GREGGI ANTUNES, LUSANIA MARIA. Transcriptome and DNA methylation changes modulated by sulforaphane induce cell cycle arrest, apoptosis, DNA damage, and suppression of proliferation in human liver cancer cells. Food and Chemical Toxicology, v. 136, FEB 2020. (16/14703-1, 17/21561-1, 17/24576-0)

MACHADO, ANA RITA THOMAZELA; TUTTIS, KATIUSKA; SANTOS, PATRICK WELLINGTON DA SILVA; AISSA, ALEXANDRE FERRO; ANTUNES, LUSANIA MARIA GREGGI. Diallyl Disulfide Induces Chemosensitization to Sorafenib, Autophagy, and Cell Cycle Arrest and Inhibits Invasion in Hepatocellular Carcinoma. PHARMACEUTICS, v. 14, n. 12, p. 18-pg., 2022-12-01. (19/08348-2, 17/24576-0)

SANTOS, PATRICK WELLINGTON; THOMAZELA MACHADO, ANA RITA; DE GRANDIS, RONE; RIBEIRO, DIEGO LUIS; TUTTIS, KATIUSKA; MORSELLI, MARCO; AISSA, ALEXANDRE FERRO; PELLEGRINI, MATTEO; GREGGI ANTUNES, LUSANIA MARIA. Effects of sulforaphane on the oxidative response, apoptosis, and the transcriptional profile of human stomach mucosa cells in vitro. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 854, JUN-JUL 2020. (16/14703-1, 17/21561-1, 17/24576-0)

TUTTIS, KATIUSKA; THOMAZELA MACHADO, ANA RITA; DE SILVA SANTOS, PATRICK WELLINGTON; GREGGI ANTUNES, LUSANIA MARIA. Sulforaphane Combined with Vitamin D Induces Cytotoxicity Mediated by Oxidative Stress, DNA Damage, Autophagy, and JNK/MAPK Pathway Modulation in Human Prostate Tumor Cells. NUTRIENTS, v. 15, n. 12, p. 18-pg., 2023-06-01. (17/24576-0)