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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of sulforaphane on the oxidative response, apoptosis, and the transcriptional profile of human stomach mucosa cells in vitro

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Author(s):
Santos, Patrick Wellington [1] ; Thomazela Machado, Ana Rita [1] ; De Grandis, Rone [2] ; Ribeiro, Diego Luis [2] ; Tuttis, Katiuska [2] ; Morselli, Marco [3] ; Aissa, Alexandre Ferro [1] ; Pellegrini, Matteo [3] ; Greggi Antunes, Lusania Maria [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo, SP - Brazil
[3] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90024 - USA
Total Affiliations: 3
Document type: Review article
Source: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 854, JUN-JUL 2020.
Web of Science Citations: 0
Abstract

Oxidative stress is a critical factor in the pathogenesis of several gastrointestinal diseases. Sulforaphane (SFN), a bioactive compound found in cruciferous vegetables, activates the redox-sensitive nuclear erythroid 2-related factor 2 (NRF2). In addition to its protective role, SFN exerts cytotoxic effects on cancer cells. However, there is a lack of information concerning the toxicity of SFN in normal cells. We investigated the effects of SFN on cell viability, antioxidant defenses, and gene expression in human stomach mucosa cells (MNP01). SFN reduced ROS formation and protected the cells against induced oxidative stress but high concentrations increased apoptosis. An intermediate SFN concentration (8 mu M) was chosen for RNA sequencing studies. We observed upregulation of genes of the NRF2 (antioxidant) pathway, the DNA damage response, and apoptosis signaling; whereas SFN downregulated cell cycle and DNA repair pathway genes. SFN may be cytoprotective at low concentrations and cytotoxic at high concentrations. (AU)

FAPESP's process: 16/14703-1 - Influence of sulforaphane, an inhibitor of histone deacetylases on the genomic instability and epigenetic mechanisms in human cell lines
Grantee:Patrick Wellington da Silva dos Santos
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/21561-1 - Effects of sulforaphane on DNA methylation patterns and transcriptome analysis in cancer and non-cancer cells
Grantee:Patrick Wellington da Silva dos Santos
Support type: Scholarships abroad - Research Internship - Master's degree
FAPESP's process: 17/24576-0 - Effects of nutraceuticals sulforaphane, diallyl disulfide and vitamin D in human cancer cell lines: Evaluation of cytotoxicity, genotoxicity, cell migration, epigenetics changes and gene expression
Grantee:Lusânia Maria Greggi Antunes
Support type: Regular Research Grants