Effects of sulforaphane on the oxidative response, apoptosis, and the transcriptional profile of human stomach mucosa cells in vitro

Author(s)

Santos, Patrick Wellington ^[1] ; Thomazela Machado, Ana Rita ^[1] ; De Grandis, Rone ^[2] ; Ribeiro, Diego Luis ^[2] ; Tuttis, Katiuska ^[2] ; Morselli, Marco ^[3] ; Aissa, Alexandre Ferro ^[1] ; Pellegrini, Matteo ^[3] ; Greggi Antunes, Lusania Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo, SP - Brazil

^[3] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90024 - USA

• Document type: Review article
• Source: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 854, JUN-JUL 2020.
• Web of Science Citations: 0

Abstract

Oxidative stress is a critical factor in the pathogenesis of several gastrointestinal diseases. Sulforaphane (SFN), a bioactive compound found in cruciferous vegetables, activates the redox-sensitive nuclear erythroid 2-related factor 2 (NRF2). In addition to its protective role, SFN exerts cytotoxic effects on cancer cells. However, there is a lack of information concerning the toxicity of SFN in normal cells. We investigated the effects of SFN on cell viability, antioxidant defenses, and gene expression in human stomach mucosa cells (MNP01). SFN reduced ROS formation and protected the cells against induced oxidative stress but high concentrations increased apoptosis. An intermediate SFN concentration (8 mu M) was chosen for RNA sequencing studies. We observed upregulation of genes of the NRF2 (antioxidant) pathway, the DNA damage response, and apoptosis signaling; whereas SFN downregulated cell cycle and DNA repair pathway genes. SFN may be cytoprotective at low concentrations and cytotoxic at high concentrations. (AU)

• FAPESP's process: 16/14703-1 - Influence of sulforaphane, an inhibitor of histone deacetylases on the genomic instability and epigenetic mechanisms in human cell lines
• Grantee: Patrick Wellington da Silva dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/21561-1 - Effects of sulforaphane on DNA methylation patterns and transcriptome analysis in cancer and non-cancer cells
• Grantee: Patrick Wellington da Silva dos Santos
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 17/24576-0 - Effects of nutraceuticals sulforaphane, diallyl disulfide and vitamin D in human cancer cell lines: Evaluation of cytotoxicity, genotoxicity, cell migration, epigenetics changes and gene expression
• Grantee: Lusânia Maria Greggi Antunes
• Support Opportunities: Regular Research Grants