Antioxidant and anti-inflammatory activities of menthol in skin wound healing

• Grant number: 15/06636-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: May 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Claudia Helena Pellizzon
• Grantee: Eduardo Oliveira de Souza
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The wound healing has been reasons to working over the years and the search for topical treatments to help in this process generated new pharmacotherapy, with large exploration of the animal products. The scar process steps described by authors are joined and receiving in general terms the following designation: inflammation, proliferation and maturation. Menthol is a terpene found in essential oils extracted from plants of the genus Mentha. This aim of this project is to evaluate the effect of menthol-based cream in skin wound healing, elucidating the mechanism of action. Male Wistar rats (n=8) will be randomly divided in five groups: cream base (control group), collagenase (positive control), and menthol-based cream at 0,25%, 0,5% or 1%. The animals will be anesthetized, and subsequently wounds in the dorsal region will be developed with the aid of a punch. Every day, the wounds will be treated, photographed and measured. The rats will be euthanized after 3, 7 or 14 days of treatment (in agreement with the phases which are described in the literature). Menthol antioxidant activity will be assessed in the skin samples, through ELISA method, by measuring the level of glutathione (GSH), and the activity of glutathione peroxidase (GPx) and reductase (GR), superoxide dismutase (SOD) and myeloperoxidase (MPO). The anti-inflammatory activity of menthol will be analyzed in the blood plasma, through ELISA's kits of cytokines TNF-±, IL-6 and IL-10. This project is linked on the post-doctoral project of the Doctor Ariane Leite Rozza (Fapesp process 2013/22202-4) and the regular support by Fapesp 2013/23340-1.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROZZA, ARIANE LEITE; BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; OLIVEIRA DE SOUZA, EDUARDO; HUSSNI, CARLOS ALBERTO; MARTINEZ, EMANUEL RICARDO MONTEIRO; NOBREGA, RAFAEL HENRIQUE; PELLIZZON, CLAUDIA HELENA. The Use of Menthol in Skin Wound Healing-Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization. PHARMACEUTICS, v. 13, n. 11, NOV 2021. (13/23340-1, 13/22202-4, 15/06636-0, 16/13307-5)