The impact of Cymbopogon martinii essential oil on the human skin microbiota and its interaction with the dermis and epidermis

• Grant number: 14/24094-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2015
• End date: May 25, 2018
• Field of knowledge: Biological Sciences - Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ary Fernandes Júnior
• Grantee: Bruna Fernanda Murbach Teles Andrade
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 15/12906-0 - The impact of Cymbopogon martinii-derived essential oils on the human skin microbiota and its interaction with the dermis and epidermis, BE.EP.PD

Abstract

The microbiota can influence physiological functions, including protection against infections, patterns of immune system reaction and willingness to autoimmune diseases. Bacterial genera such as Staphylococcus, Propionibacterium, Micrococcus and Corynebacterium are found in the microbiota of human skin, but the skin of the face and back 50% of the total microbiota is represented by a micro-organism, the bacterium Propionibacterium acnes. There are strains of P. acnes associated with acne, while others have been associated with opportunistic infections. The epidermis has the keratinocyte cells to a greater number and dermis contains immune cells such as T cells, also found in the infiltrate surrounding the acne. The aim of this research is to evaluate the impact of Cymbopogon martinii essential oil and it´s major compound, geraniol on the skin microbiota and to examine whether these compounds are able to modify the immune stimulation caused by P. acnes. For this, we will use P. acnes strains of subtype IA and II, we will determine the antibacterial activity of C. martini essential oil and geraniol for P. acnes in vitro, changes in cell viability and phenotypic changes and responses in the transcriptome and proteome triggered. Analyze the immunomodulatory activity and cytotoxicity of the essential oil and geraniol in keratinocytes and lymphocytes in the absence or presence of P. acnes. Determine the changes in the human skin microbiota after topical administration of the compounds through metagenomics. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MURBACH TELES ANDRADE, BRUNA FERNANDA; BARBOSA, LIDIANE NUNES; BERGAMO ALVES, FERNANDA CRISTINA; PEREIRA MARQUES, ANA FLAVIA; ALBANO, MARIANA; MORES RALL, VERA LUCIA; BRUEGGEMANN, HOLGER; FERNANDES JUNIOR, ARY. The impact of Cymbopogon martinii essential oil on Cutibacterium (formerly Propionibacterium) acnes strains and its interaction with keratinocytes. Journal of Pharmacy and Pharmacology, v. 70, n. 12, p. 1688-1699, DEC 2018. (15/12906-0, 14/24094-7)