Plasma circulating microRNA profile as a biomarker in the early diagnosis and prognosis of chronic thromboembolic pulmonary hypertension

• Grant number: 15/06708-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2015
• End date: June 30, 2016
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Alexandre Todorovic Fabro
• Grantee: Heloísa Resende Cruvinel
• Host Institution: Hospital das Clínicas. Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The Chronic Thromboembolic Pulmonary Hypertension (CTEPH) is characterized by intraluminal thrombus organization and pulmonary remodeling, increased pulmonary vascular resistence.The consequence is progressive pulmonary arterial hypertension (PAH) and right heart failure with a 3-year mortality rate of 90%.Simultaneously, muscular and elastic pulmonary small vessels have been obstructed and they have also been related to the hypoxia and endothelial disfunction.All these physiopathologic processes were targeted by altered molecular signaling pathways associated with a progressive vacular injury, which is related to change of gene expression and their final proteins. The pos transcriptional control may be induced by microRNAs, which acts like endogen repressor of pos transcriptional gene silencing, blocking of protein translation by RNA degradation. This regulatory mechanism is critical for several biological processes such as cellular proliferation, apoptosis, angiogenesis, fibrogenesis and cellular differentiation. However, some microRNAs are actively released to blood flow after biological stimulus and / or death cell-derived, then they are called circulating microRNA and may be a better candidate to diagnostic and prognostic biomarker of CTEPH. Our aims is to do sequencing of circulating miRNAs and to identify novel markers predicting clinical outcome.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FABRO, ALEXANDRE TODOROVIC; MACHADO-RUGOLO, JULIANA; BALDAVIRA, CAMILA MACHADO; PRIETO, TABATHA GUTIERREZ; FARHAT, CECILIA; ROTEA MANGONE, FLAVIA REGINA; BATAH, SABRINA SETEMBRE; CRUVINEL, HELOISA RESENDE; ALDA, MAIARA ALMEIDA; MONTEIRO, JHONATAS SIRINO; et al. Circulating Plasma miRNA and Clinical/Hemodynamic Characteristics Provide Additional Predictive Information About Acute Pulmonary Thromboembolism, Chronic Thromboembolic Pulmonary Hypertension and Idiopathic Pulmonary Hypertension. FRONTIERS IN PHARMACOLOGY, v. 12, MAY 28 2021. (15/06387-0, 18/20403-6, 15/06708-0)