Energy expenditure evaluation and metabolic characterization of brown and subcutaneous adipose tissues in obese mice supplemented with green tea

• Grant number: 15/07024-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2015
• End date: April 26, 2016
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Rosemari Otton
• Grantee: Gustavo Tolentino Nascimento dos Santos
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil

Abstract

The number of overweight and obesity increases dramatically around the world. The imbalance between caloric intake and energy expenditure is the main cause of obesity, defined by WHO by excessive or abnormal accumulation of fat, which can cause damage to health. As a result we observe an increase directly or indirectly related to the disease overweight such as diabetes mellitus type 2 (DM2), cardiovascular diseases, among others. A tissue with high regulatory potential and now considered essential for metabolic homeostasis is the brown adipose tissue (BAT) because of its energy storage characteristics, which under appropriate stimulus is directed to the production of heat, thus contributing to the loss of weight by increasing energy expenditure. Many studies evaluate foods or compounds that may stimulate energy expenditure and thus represent an adjuvant in the treatment of obesity, including the green tea. The objective of this study is to analyze the effect of supplementation with green tea in brown and subcutaneous adipose tissue of mice induced to obesity by cafeteria diet. The animals receive diet for 4 weeks (1 month) and thereafter start the gavage with green tea extract (500 mg / kg) for 12 weeks (3 months) in addition to cafeteria diet. Later euthanasia and obtaining tissue will be held. Total RNA from the cells is extracted by using the Tri-reagent and subsequent realization of real-time PCR (Polymerase Chain Reaction) for evaluating PPARc, LXR±, CD36, LPL, FAS, UCP1, PGC1±, Cidea, Adr²3, PRDM16, CPT1, DIO2 and AP2. With this study we hope to contribute to the understanding of the effects of green tea on metabolic profile of BAT and subcutaneous adipose tissue and encourage further research on the effect of bioactive compounds in the fight against obesity and its complications.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOLIN, ANAYSA PAOLA; BATISTA SOUSA-FILHO, CELSO PEREIRA; NASCIMENTO DOS SANTOS, GUSTAVO TOLENTINO; FERREIRA, LETICIA TORRES; MARTINS DE ANDRADE, PAULA BRESCIANI; MIGLIORINI FIGUEIRA, ANA CAROLINA; HELENO BATISTA, FERNANDA APARECIDA; OTTON, ROSEMARI. Adipogenic commitment induced by green tea polyphenols remodel adipocytes to a thermogenic phenotype. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 83, SEP 2020. (13/22293-0, 15/07024-8, 11/19216-8, 12/20415-8)