Analysis of the genes expression related to energy expenditure and lipid metabolism in individuals with grade III obesity before and after supplementation with green tea

• Grant number: 14/00669-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2014
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Carla Barbosa Nonino
• Grantee: Driele Cristina Gomes Quinhoneiro
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Obesity is considered an epidemic disease, represented by the excessive accumulation of adipose tissue and is related to several environmental and genetic causes. Studies indicate that the energy balance, determined by food intake and energy expended, seems to depend on 40 % of the genetic heritage. The aim of this study is to check the expression of genes ADBR3, CPUs, PLIN1, PPAR2 link to obesity, energy expenditure and lipid metabolism in subcutaneous adipose abdominal tissue and associated with anthropometric, dietary and biochemical variables and resting metabolic rate in individuals grade III obese patients before and after supplementation with green tea extract (Camellia simensi). The sample will consist of 20 subjects divided into two groups: control group with 10 women with normal-weight and intervention group with 10 women with morbid obesity, both aged between 18 and 60 years. Patients will undergo anthropometric measurements (weight, height and body mass index) and body composition (bioelectrical impedance method), the resting metabolic rate (indirect calorimetry test), the assessment of dietary intake (24-hour recall), the biochemical evaluation (blood glucose, lipid profile, blood count, minerals, proteins, albumin and hepatic biomarkers) and analysis of gene expression by the method of quantitative real time PCR. After 8 weeks of supplementation with green tea intervention group will be re-evaluated and the results compared with the control group. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES QUINHONEIRO, DRIELE CRISTINA; NICOLETTI, CAROLINA FERREIRA; SOUZA PINHEL, MARCELA AUGUSTA; NORONHA, NATALIA YUMI; MORENO BRAGA, CAMILA BITU; PARENTI OLIVEIRA, BRUNO AFFONSO; CORTES-OLIVEIRA, CRISTIANA; OLIVEIRA, WANDERLEY PEREIRA; SALGADO JUNIOR, WILSON; MARCHINI, JULIO SERGIO; et al. Green tea supplementation upregulates uncoupling protein 3 expression in severe obese women adipose tissue but does not promote weight loss. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, v. 69, n. 8, p. 995-1002, NOV 17 2018. (13/08916-4, 14/00669-0)

NONINO, CARLA B.; PINHANELLI, VITOR CARESSATO; NORONHA, NATALIA Y.; QUINHONEIRO, DRIELE C. G.; SOUZA PINHEL, MARCELA A.; DE OLIVEIRA, BRUNO A. P.; MARCHINI, JULIO SERGIO; NICOLETTI, CAROLINA FERREIRA. Green tea supplementation promotes leukocyte telomere length elongation in obese women. NUTRICION HOSPITALARIA, v. 35, n. 3, p. 570-575, MAY-JUN 2018. (13/08916-4, 14/00669-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

QUINHONEIRO, Driele Cristina Gomes. Analysis of gene expression related energy and lipid metabolism in women with obesity grade III after decaffeinated green tea extract supplementation. 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.