Analysis of the expression of genes related to energy expenditure and lipid metabolism in individuals with grade III obesity before and after interventions for weight loss

• Grant number: 13/08916-4
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2014 - June 30, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Carla Barbosa Nonino
• Grantee: Carla Barbosa Nonino
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Obesity is considered an epidemic disease, represented by the excessive accumulation of adipose tissue and is linked to various genetic and environmental causes. Studies indicate that the energy balance, determined by energy intake and energy expended, seems to depend on 40% of genetic inheritance. The aim of this study is to check the expression of genes ADBR3, UCPs, PLIN, PPARG2 related to obesity, energy expenditure and lipid metabolism in abdominal subcutaneous adipose tissue and associated with anthropometric variables, the resting metabolic rate, dietary and biochemical in grade III obese patients before and after three different interventions for weight loss. Will be selected for the study 40 women with grade III obesity aged 18 to 60 years which will be randomized into four groups: Group 1 - Dietary Intervention, Group 2 - Supplementation with Green Tea, Group 3 - Bariatric Surgery and Group 4 - Control. Patients will undergo nutritional assessment, body composition analysis, resting metabolic rate, food intake analysis, socio-demographic questionnaire and collecting biological material collection (peripheral blood and / or subcutaneous adipose tissue) by specialized professional for genetic analysis and biochemistry. After 8 weeks for G1 and G2 and G3 six months for all procedures are performed again. Statistical analysis will be used descriptive statistics with the values presented as mean and standard deviation. Will be applied t test, chi-square or Fisher and admitted to the significance level P <0.05. All analyzes performed in software Statistical Package for Social Science (SPSS versão 17.0 [Inc. Chicago. IL]). (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORTES-OLIVEIRA, C.; NICOLETTI, C. F.; DE SOUZA PINHEL, MA; DE OLIVEIRA, BAP; QUINHONEIRO, DCG; NORONHA, N. Y.; MARCHINI, J. S.; DA SILVA, W. A. JUNIOR; S, W. JUNIOR; NONINO, C. B.. UCP2 expression is associated with weight loss after hypocaloric diet intervention. European Journal of Clinical Nutrition, v. 71, n. 3, SI, p. 402-406, MAR 2017. (13/08916-4)

GOMES QUINHONEIRO, DRIELE CRISTINA; NICOLETTI, CAROLINA FERREIRA; SOUZA PINHEL, MARCELA AUGUSTA; NORONHA, NATALIA YUMI; MORENO BRAGA, CAMILA BITU; PARENTI OLIVEIRA, BRUNO AFFONSO; CORTES-OLIVEIRA, CRISTIANA; OLIVEIRA, WANDERLEY PEREIRA; SALGADO JUNIOR, WILSON; MARCHINI, JULIO SERGIO; et al. Green tea supplementation upregulates uncoupling protein 3 expression in severe obese women adipose tissue but does not promote weight loss. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, v. 69, n. 8, p. 995-1002, NOV 17 2018. (13/08916-4, 14/00669-0)

NATÁLIA Y NORONHA; MARCELA A SOUZA PINHEL; CAROLINA F NICOLETTI; DRIELE C QUINHONEIRO; VITOR C PINHANELLI; BRUNO A P DE OLIVEIRA; CRISTINA CORTES-OLIVEIRA; HEITOR B P DELFINO; LETÍCIA S WOLF; FABIANI G FRANTZ; et al. Green tea supplementation improves oxidative stress biomarkers and modulates IL-6 circulating levels in obese women. NUTRICION HOSPITALARIA, v. 36, n. 3, p. 583-588, 2020-02-10. (13/08916-4)

NONINO, CARLA B.; PINHANELLI, VITOR CARESSATO; NORONHA, NATALIA Y.; QUINHONEIRO, DRIELE C. G.; SOUZA PINHEL, MARCELA A.; DE OLIVEIRA, BRUNO A. P.; MARCHINI, JULIO SERGIO; NICOLETTI, CAROLINA FERREIRA. Green tea supplementation promotes leukocyte telomere length elongation in obese women. NUTRICION HOSPITALARIA, v. 35, n. 3, p. 570-575, MAY-JUN 2018. (13/08916-4, 14/00669-0)

DE OLIVEIRA, CRISTIANA CORTES; NICOLETTI, CAROLINA FERREIRA; DE SOUZA PINHEL, MARCELA AUGUSTA; PARENTI DE OLIVEIRA, BRUNO AFFONSO; GOMES QUINHONEIRO, DRIELE CRISTINA; NORONHA, NATALIA YUMI; FASSINI, PRISCILA GIACOMO; MARCHINI, JULIO SERGIO; DA SILVA JUNIOR, WILSON ARM; SALGADO JUNIOR, WILSON; et al. Influence of expression of UCP3, PLIN1 and PPARG2 on the oxidation of substrates after hypocaloric dietary intervention. Clinical Nutrition, v. 37, n. 4, p. 1383-1388, AUG 2018. (13/08916-4)

OLIVEIRA, BRUNO A. P.; PINHEL, MARCELA A. S.; NICOLETTI, CAROLINA F.; OLIVEIRA, CRISTIANA C.; QUINHONEIRO, DRIELE C. G.; NORONHA, NATALIA Y.; MARCHINI, JULIO S.; MARCHRY, ANA J.; JUNIOR, WILSON S.; NONINO, CARLA B.. UCP1 and UCP3 Expression Is Associated with Lipid and Carbohydrate Oxidation and Body Composition. PLoS One, v. 11, n. 3, MAR 9 2016. (13/08916-4, 13/06563-7)

CORTES-OLIVEIRA, C.; NICOLETTI, C. F.; DE SOUZA PINHEL, MA; DE OLIVEIRA, BAP; QUINHONEIRO, DCG; NORONHA, N. Y.; MARCHINI, J. S.; DA SILVA, W. A. JUNIOR; S, W. JUNIOR; NONINO, C. B.. UCP2 expression is associated with weight loss after hypocaloric diet intervention. European Journal of Clinical Nutrition, v. 71, n. 3, p. 5-pg., 2017-03-01. (13/08916-4)

C. F. NICOLETTI; H. B. P. DELFINO; M. A. S. PINHEL; N. Y. NORONHA; V. C. PINHANELLI; D. C. G. QUINHONEIRO; B. A. P. OLIVEIRA; J. S. MARCHINI; C. B. NONINO. Impact of green tea epigallocatechin-3-gallate on HIF1-α and mTORC2 expressionin obese women: anti-cancer and anti-obesity effects?. NUTRICION HOSPITALARIA, v. 36, n. 2, p. 315-320, 2020-01-20. (13/08916-4)

NICOLETTI, CAROLINA FERREIRA; PEREIRA DELFINO, HEITOR BERNARDES; SOUZA PINHEL, MARCELA AUGUSTA; NORONHA, NATALIA YUMI; PINHANELLI, VITOR CARESSATO; GOMES QUINHONEIRO, DRIELE CRISTINA; PARENTI OLIVEIRA, BRUNO AFFONSO; MARCHINI, JULIO SERGIO; NONINO, CARLA BARBOSA. Impact of green tea epigallocatechin-3-gallate on HIF1-alpha and mTORC2 expression in obese women: anti-cancer and anti-obesity effects?. NUTRICION HOSPITALARIA, v. 36, n. 2, p. 315-320, MAR-APR 2019. (13/08916-4)

FERREIRA NICOLETTI, CAROLINA; BARBOSA NONINO, CARLA; PARENTI DE OLIVEIRA, BRUNO AFFONSO; DE SOUZA PINHEL, MARCELA AUGUSTA; LUISA MANSEGO, MARIA; IGNACIO MILAGRO, FERMIN; ANGELES ZULET, MARIA; ALFREDO MARTINEZ, JOSE. DNA Methylation and Hydroxymethylation Levels in Relation to Two Weight Loss Strategies: Energy-Restricted Diet or Bariatric Surgery. OBESITY SURGERY, v. 26, n. 3, p. 603-611, MAR 2016. (13/08916-4, 14/04902-1)

PARENTI DE OLIVEIRA, BRUNO AFFONSO; DE SOUZA PINHEL, MARCELA AUGUSTA; NICOLETTI, CAROLINA FERREIRA; DE OLIVEIRA, CRISTIANA CORTES; GOMES QUINHONEIRO, DRIELE CRISTINA; NORONHA, NATALIA YUMI; FASSINI, PRISCILA GIACOMO; DA SILVA JUNIOR, WILSON ARAUJO; SALGADO JUNIOR, WILSON; NONINO, CARLA BARBOSA. UCP2 and PLIN1 Expression Affects the Resting Metabolic Rate and Weight Loss on Obese Patients. OBESITY SURGERY, v. 27, n. 2, p. 343-348, FEB 2017. (13/06563-7, 13/08916-4)