Analysis of the expression of genes related to energy expenditure of grade III obese before and after bariatric surgery

• Grant number: 13/06563-7
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2013
• Effective date (End): March 31, 2015
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Carla Barbosa Nonino
• Grantee: Bruno Affonso Parenti de Oliveira
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Obesity is considered an epidemic disease, represented by the excessive accumulation of adipose tissue and it is related to several genetic and environmental causes. Studies indicate that the energy balance, determined by energy intake and energy expended, seems to depend on 40% of genetic inheritance. The main objective of the study is check the expression of genes ADBR3, CPUs, PLIN, PPAR”2, related to obesity in subcutaneous adipose tissue and associate it with measures of resting metabolic rate in obese individuals grade III. The sample will be composed of 20 individuals, a control group of 10 women with normal weight and another group with 10 obese individuals grade III of mixed population, females, aged between 18 and 60 years, candidates for bariatric surgery, with undergoing regular monitoring in Bariatric Surgery Clinic HCFMRP-USP. The classification of nutritional status will be made by the Body Mass Index (BMI), and, thereafter, will be submitted to evaluation of body composition by the bioelectrical impedance analysis method of resting metabolic rate (RMR), by means of indirect calorimetry, and analysis of gene expression by the quantitative PCR method in real time. After six months the participants submitted to bariatric surgery will be reassessed and the results compared between the control group and pre and post surgical procedure. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, BRUNO A. P.; PINHEL, MARCELA A. S.; NICOLETTI, CAROLINA F.; OLIVEIRA, CRISTIANA C.; QUINHONEIRO, DRIELE C. G.; NORONHA, NATALIA Y.; MARCHINI, JULIO S.; MARCHRY, ANA J.; JUNIOR, WILSON S.; NONINO, CARLA B.. UCP1 and UCP3 Expression Is Associated with Lipid and Carbohydrate Oxidation and Body Composition. PLoS One, v. 11, n. 3, MAR 9 2016. (13/08916-4, 13/06563-7)

PARENTI DE OLIVEIRA, BRUNO AFFONSO; DE SOUZA PINHEL, MARCELA AUGUSTA; NICOLETTI, CAROLINA FERREIRA; DE OLIVEIRA, CRISTIANA CORTES; GOMES QUINHONEIRO, DRIELE CRISTINA; NORONHA, NATALIA YUMI; FASSINI, PRISCILA GIACOMO; DA SILVA JUNIOR, WILSON ARAUJO; SALGADO JUNIOR, WILSON; NONINO, CARLA BARBOSA. UCP2 and PLIN1 Expression Affects the Resting Metabolic Rate and Weight Loss on Obese Patients. OBESITY SURGERY, v. 27, n. 2, p. 343-348, FEB 2017. (13/06563-7, 13/08916-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Bruno Affonso Parenti de. Analysis of gene expression related to energy expenditure of obese grade III before and after bariatric surgery. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.