Analysis of FosB and JunB expression and interaction partners after stimulation of FGF-2 tumor cells

• Grant number: 15/14522-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2015
• End date: December 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Julia Pinheiro Chagas da Cunha
• Grantee: Jaqueline Neves Moreno
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 11/22619-7 - Nuclei and chromatin alterations through cell cycle and senescence in mammalian cells, AP.JP

Abstract

The fibroblast growth factor - 2 (FGF2) acts as a mitogenic agent, but in some cellular contexts, has antiproliferative functions and may be an important tumor suppressor. In mice tumor cells (lineage Y1), FGF2 acts by inhibiting the transition G0 / G1 to S and irreversibly blocks the cells in G2 / M. Quantitative proteomic analyzes performed by our group identified several differentially expressed proteins after treatment of these cells with FGF2. Among them, we identified the transcription factors FosB and JunB belonging to the FOS and JUN families, respectively. Western blotting analysis confirmed that these proteins are differentially expressed upon treatment with FGF2 compared to cells stimulated only with fetal bovine serum. Thus, the main objective of this project is to better understand the role of fosB and junB upon FGF2stimulus, including, I. characterize the fosB and junB expression profile after FGF2 stimulation, as well as the proteins directly (or indirectly) associated with its activation and II. identify interaction partners of these proteins by co-immunoprecipitation assays. We believe these results will allow a better understanding of the molecular components responsible for the antiproliferative effects of FGF2.