Large-scale analysis of protein and biological networks construction focusing on B chromosome

• Grant number: 15/11485-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2015
• End date: August 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: César Martins
• Grantee: Rafael Takahiro Nakajima
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The B chromosomes occur in about 15% of eukaryotic species, being primarily composed of repeated DNAs and heterochromatin. Some studies have been performed analysis of distribution, frequency, inheritance mechanisms, structure and origin of these chromosomes. Recently, large scale genomic analysis have been used to elucidate questions about the supernumerary chromosomes. Cichlid fishes are very interesting models in science, since many species have undergone rapid and extensive process of adaptive radiation. In some species of these group, as Astatotilapia latifasciata, it was described the presence of B chromosomes. Our objective is to characterize the profile of protein expression in specific tissues using A. latifasciata as a model. The methodology consists in using the liquid chromatography high performance coupled with mass spectrometry (LC-MS / MS) to conduct a functional analysis of B chromosome presence, elucidating its influence in specific metabolic pathways. Furthermore, we will integrate these data with the RNA Seq results using biological networks, enabling a better understanding of the influence of B chromosome presence in cell. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NAKAJIMA, Rafael Takahiro. Large scale analyzes of proteins and biological network construction focusing on B chromosome studies. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.