Evaluation of electrophoretic profile in the soro of dogs (Canis familiaris) naturally infected with canine distemper virus

• Grant number: 15/05338-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2015
• End date: January 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Márcia Ferreira da Rosa Sobreira
• Grantee: Mayara Chicon Rosente
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Canine distemper is a contagious disease important, with high rate of mortality and multisystem involvement. The demyelinating leukoencephalitis is the main neuropathological changes observed, and its little known pathogenesis. The breakdown of the blood brain barrier (BBB) with viral penetration and leukocyte infiltration are crucial aspects in the development of demyelinating encephalitis. Thus, the present study has as main scope to establish the profile of serum proteins dogs with distemper in neurological phase, through electrophoresis in polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE), in the serum of healthy dogs - group control (GCON, n = 10) and naturally infected dogs by canine distemper virus, the neurological stage of the disease - distemper group (Gcin, n = 10) with the aim of evaluating the serum proteins and their role in the pathogenesis of the disease.