Effect of low-level laser therapy on pulmonary inflammation in an experimental model of chronic obstructive pulmonary disease (COPD) induced by cigarette smoking: laser dosimetry studies

• Grant number: 15/13486-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2015
• End date: July 31, 2017
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Ana Paula Ligeiro de Oliveira
• Grantee: Cintia Estefano Alves
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 12/16498-5 - Effect of low level laser therapy in experimental models of pulmonary chronic diseases, AP.JP

Abstract

Currently the Chronic Obstructive Pulmonary Disease (COPD) has a high prevalence and a high economic and social cost. In this context, several experimental models have been proposed, aiming at the discovery of new therapeutic approaches. Accordingly, the therapy with Low Power Laser (LBP) is a relatively new effective therapy, with very low cost and no side effects. In this project we aim to study some parameters in animals with COPD undergoing therapies with LBP (15 days before the experiment with 1J, 3 J, 5J and 7.5 J/cm2). The protocol used for the induction of COPD consists in submitting C57BL / 6 mice for 75 days (2 times / day) to inhaled cigarette smoke. On day 76, the animals will be sacrificed for morphologic and functional analysis of the lung. We will study structural and functional parameters, such as the release of cytokines and chemokines (IL-1², IL-6, IL-10, IL-12, IFN-g, TNF-±, TGF-², KC) and accumulation of mucus and collagen. We are also interested in evaluating the suppression of NF-kB signaling as well as MMP-9 and MMP-12.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; et al. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018. (12/16498-5, 15/13486-4, 12/15165-2, 14/14604-8, 15/23152-6)

MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; et al. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2018, p. 8-pg., 2018-01-01. (12/16498-5, 14/14604-8, 15/23152-6, 15/13486-4, 12/15165-2)