Effects of environment and lifestyle on asthma and chronic obstructive pulmonary disease: studies with experimental animals and people with asthma

• Grant number: 09/53904-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: November 01, 2010
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Milton de Arruda Martins
• Grantee: Milton de Arruda Martins
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant(s): 14/12031-0 - European Respiratory Society Annual Congress 2014, AR.EXT; 13/05356-8 - American Thoracic Society 2013 International Conference, AR.EXT; 12/03397-6 - American Thoracic Society International Conference 2012, AR.EXT
• Associated scholarship(s): 14/16511-7 - Comparison of continuous and interval training on the perception of physical effort in health related quality of life and level of physical activity in asthma patients, BP.MS; 12/09932-0 - Effects of regular aerobic exercise in mice exposed to cigarette smoke: temporal profile of the inflammatory response and oxidative stress, BP.PD

Abstract

Asthma and Chronic Obstructive Pulmonary Disease (COPD) are very important health problems. The purpose of this project is to study mechanisms related to environmental and lifestyle influences involved in the modulation of inflammation, hyperresponsiveness and pulmonary remodeling in asthma and COPD. We will study experimental models of chronic allergic pulmonary inflammation in mice and guinea pigs and murine models of COPD induced by exposure to cigarette smoke and to administration of elastase. We will use experimental models of stress, physical training, exposure to atmospheric pollution, administration of endotoxin and cigarette smoke. We will study pulmonary mechanics, pulmonary responsiveness to contractile agonists, inflammatory cell profile of bronchoalveolar lavage fluid (BAL), levels of cytokines and other inflammatory mediators in BAL and pulmonary tissue, using ELISA and immunohistochemistry and perform histologic evaluation of pulmonary tissue, measurements of pulmonary remodeling, and molecular biology techniques to evaluate the expression of certain genes. We will develop an equipment to expose experimental animals to cigarette smoke. We will study, in adults with persistent asthma, the effects of regular exercise in asthma control, in the severity of airway inflammation, psychosocial factors and quality of life. We will study the mechanisms of Exercise-Induced Bronchospasm (EIB) in children with clinically stable persistent asthma, evaluating quality of life scores and airway inflammation measured with induced sputum and breath condensate of expired air in asthmatic children with and without EIB. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HIZUME, DEBORAH C.; TOLEDO, ALESSANDRA C.; MORIYA, HENRIQUE T.; SARAIVA-ROMANHOLO, BEATRIZ M.; ALMEIDA, FRANCINE M.; ARANTES-COSTA, FERNANDA M.; VIEIRA, RODOLFO P.; DOLHNIKOFF, MARISA; KASAHARA, DAVID ITIRO; MARTINS, MILTON A.. Cigarette smoke dissociates inflammation and lung remodeling in OVA-sensitized and challenged mice. Respiratory Physiology & Neurobiology, v. 181, n. 2, p. 167-176, APR 30 2012. (09/53904-9)

FRANCA-PINTO, ANDREZZA; MENDES, FELIPE A. R.; DE CARVALHO-PINTO, REGINA MARIA; AGONDI, ROSANA CAMARA; CUKIER, ALBERTO; STELMACH, RAFAEL; SARAIVA-ROMANHOLO, BEATRIZ M.; KALIL, JORGE; MARTINS, MILTON A.; GIAVINA-BIANCHI, PEDRO; et al. Aerobic training decreases bronchial hyperresponsiveness and systemic inflammation in patients with moderate or severe asthma: a randomised controlled trial. THORAX, v. 70, n. 8, p. 732-739, AUG 2015. (09/53904-9)

DA SILVA, RONALDO APARECIDO; ALMEIDA, FRANCINE MARIA; OLIVO, CLARICE ROSA; SARAIVA-ROMANHOLO, BEATRIZ MANGUEIRA; PERINI, ADENIR; MARTINS, MILTON ARRUDA; FERNANDES CARVALHO, CELSO RICARDO. Comparison of the Effects of Aerobic Conditioning Before and After Pulmonary Allergic Inflammation. Inflammation, v. 38, n. 3, p. 1229-1238, JUN 2015. (09/53904-9)

SILVA, R. A.; ALMEIDA, F. M.; OLIVO, C. R.; SARAIVA-ROMANHOLO, B. M.; MARTINS, M. A.; CARVALHO, C. R. F.. Exercise reverses OVA-induced inhibition of glucocorticoid receptor and increases anti-inflammatory cytokines in asthma. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 26, n. 1, p. 82-92, JAN 2016. (09/53904-9)

DE GENARO, ISABELLA SANTOS; DE ALMEIDA, FRANCINE MARIA; HIZUME-KUNZLER, DEBORAH CAMARGO; MORIYA, HENRIQUE TAKACHI; SILVA, RONALDO APARECIDO; GONCALVES CRUZ, JOAO CARLOS; LOPES, RENAN BOEIRA; RIGHETTI, RENATO FRAGA; VIEIRA, RODOLFO DE PAULA; SAIKI, MITIKO; et al. Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice (vol 8, 12636, 2018). SCIENTIFIC REPORTS, v. 8, p. 1-pg., 2018-12-12. (09/53904-9, 12/15165-2)

FLORENCIO, ARIANA CORREA; DE ALMEIDA, ROBSON S.; ARANTES-COSTA, FERNANDA M.; SARAIVA-ROMANHOLO, BEATRIZ M.; DURAN, ADRIANA F.; SASAKI, SERGIO D.; MARTINS, MILTON A.; LOPES, FERNANDA D. T. Q. S.; TIBERIO, IOLANDA F. L. C.; LEICK, EDNA A.. Effects of the serine protease inhibitor rBmTI-A in an experimental mouse model of chronic allergic pulmonary inflammation (vol 9, 12624, 2019). SCIENTIFIC REPORTS, v. 9, p. 1-pg., 2019-11-13. (09/53904-9)

SUEHIRO, CAMILA LIYOKO; DE TOLEDO-ARRUDA, ALESSANDRA CHOQUETA; VIEIRA, RODOLFO DE PAULA; DE ALMEIDA, FRANCINE MARIA; OLIVO, CLARICE ROSA; MARTINS, MILTON DE ARRUDA; LIN, CHIN JIA. A possible association between fructose consumption and pulmonary emphysema. SCIENTIFIC REPORTS, v. 9, JUN 27 2019. (09/53904-9)

SARAIVA-ROMANHOLO, BEATRIZ MANGUEIRA; DE GENARO, ISABELLA SANTOS; DE ALMEIDA, FRANCINE MARIA; FELIX, SORAIA NOGUEIRA; COTTES LOPES, MARINA RIBEIRO; AMORIM, THAIS SANTOS; VIEIRA, RODOLFO PAULA; ARANTES-COSTA, FERNANDA MAGALHAES; MARTINS, MILTON ARRUDA; LOPES CALVO TIBERIO, IOLANDA DE FATIMA; et al. Exposure to Sodium Hypochlorite or Cigarette Smoke Induces Lung Injury and Mechanical Impairment in Wistar Rats. Inflammation, v. 45, n. 4, p. 20-pg., 2022-05-02. (09/53904-9, 18/06088-0)

RODRIGUES MENDES, FELIPE A.; TEIXEIRA, RENATA NAKATA; MARTINS, MILTON A.; CUKIER, ALBERTO; STELMACH, RAFAEL; MEDEIROS, WLADIMIR MUSETTI; CARVALHO, CELSO R. F.. The relationship between heart rate and VO2 in moderate-to-severe asthmatics. JOURNAL OF ASTHMA, JULY 2019. (09/53904-9)

DE GENARO, ISABELLA SANTOS; DE ALMEIDA, FRANCINE MARIA; HIZUME-KUNZLER, DEBORAH CAMARGO; MORIYA, HENRIQUE TAKACHI; SILVA, RONALDO APARECIDO; GONCALVES CRUZ, JOAO CARLOS; LOPES, RENAN BOEIRA; RIGHETTI, RENATO FRAGA; VIEIRA, RODOLFO DE PAULA; SAIKI, MITIKO; et al. Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice. SCIENTIFIC REPORTS, v. 8, AUG 22 2018. (12/15165-2, 09/53904-9)

TOLEDO-ARRUDA, ALESSANDRA C.; VIEIRA, RODOLFO P.; GUARNIER, FLAVIA A.; SUEHIRO, CAMILA L.; CALEMAN-NETO, AGOSTINHO; OLIVO, CLARICE R.; ARANTES, PETRA M. M.; ALMEIDA, FRANCINE M.; LOPES, FERNANDA D. T. Q. S.; RAMOS, ERCY M. C.; et al. Time-course effects of aerobic physical training in the prevention of cigarette smoke-induced COPD. Journal of Applied Physiology, v. 123, n. 3, p. 674-683, SEP 2017. (12/15165-2, 12/09932-0, 09/53904-9)

EVARISTO, KAREN B.; SACCOMANI, MILENE G.; MARTINS, MILTON A.; CUKIER, ALBERTO; STELMACH, RAFAEL; RODRIGUES, MARCOS R.; SANTAELLA, DANILO F.; CARVALHO, CELSO R. F.. Comparison between breathing and aerobic exercise on clinical control in patients with moderate-to-severe asthma: protocol of a randomized trial. BMC PULMONARY MEDICINE, v. 14, OCT 17 2014. (09/53904-9)

OLIVO, CLARICE R.; MIYAJI, ELIANE N.; OLIVEIRA, MARIA LEONOR S.; ALMEIDA, FRANCINE M.; LOURENCO, JULIANA D.; ABREU, RODRIGO M.; ARANTES, PETRA M. M.; LOPES, FERNANDA D. T. Q. S.; MARTINS, MILTON A.. Aerobic exercise attenuates pulmonary inflammation induced by Streptococcus pneumoniae. Journal of Applied Physiology, v. 117, n. 9, p. 998-1007, NOV 1 2014. (09/53904-9)

SILVA, R. A.; ALMEIDA, F. M.; OLIVO, C. R.; SARAIVA-ROMANHOLO, B. M.; MARTINS, M. A.; CARVALHO, C. R. F.. Airway remodeling is reversed by aerobic training in a murine model of chronic asthma. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 25, n. 3, p. e258-e266, JUN 2015. (09/53904-9)

RODRIGUES MENDES, FELIPE A.; TEIXEIRA, RENATA NAKATA; MARTINS, MILTON A.; CUKIER, ALBERTO; STELMACH, RAFAEL; MEDEIROS, WLADIMIR MUSETTI; CARVALHO, CELSO R. F.. The relationship between heart rate and VO2 in moderate-to-severe asthmatics. JOURNAL OF ASTHMA, v. 57, n. 7, p. 9-pg., 2019-07-02. (09/53904-9)