Evaluation of the Bisphenol-A carcinogenic effect in the mammary gland of Mongolian Gerbil (Merionis unguiculatus)

• Grant number: 15/01548-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2015
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Sebastião Roberto Taboga
• Grantee: Ellen Cristina Rivas Leonel
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 16/22947-8 - Techniques of female reproductive tissue morphology and viability assessment as tools for enhancing cancer research success, BE.EP.DR

Abstract

The development of new treatments for diseases related to mammary gland depends on knowledge of morphology, physiology and breast pathology. The gerbils (Meriones unguiculatus) have been widely used in scientific experiments, since they are relatively easy to handle, robust and have small costs. This project aims to characterize morphologically normal mammary gland of Mongolian gerbils and to evaluate the carcinogenic effect of bisphenol A by morphological and immunohistochemical analysis to determine early-tumoral lesions. For the step of morphological characterization, animals in puberty, pregnant, in lactation and senile will be used. The mammary glandular tissue will be stained with hematoxylin and eosin, Gomori reticulin and PAS. Also, immunohistochemical reactions for ER-alfa, ER-beta and progesterone receptors and alfa-actin will be performed. For the carcinogenic effect of bisfenol A in the mammary gland, the animals will be treated during pregnancy and lactation with different doses of bisphenol A or estrogen. From the females born, one group will be treated with carcinogen N-nitroso-N-methyl-urea and the other group will be maintained as control. The pre-neoplastic cell damage will be assessed by staining with hematoxylin and eosin, PAS and the immunohistochemical reactions for the presence of Ki67 and PCNA antigens and ER-alfa, ER-beta and progesterone receptors. In addition, we will analyze other organs for the presence of metastases. At the time of euthanasia, blood will also be collected for serum estradiol dosage.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIVAS LEONEL, ELLEN CRISTINA; PEGORIN CAMPOS, SILVANA GISELE; BARALDI BEDOLO, CAROLINA MARQUES; ALVES GUERRA, LUIZ HENRIQUE; LEITE VILAMAIOR, PATRICIA SIMONE; CALMON, MARILIA FREITAS; RAHAL, PAULA; AMORIM, CHRISTIANI ANDRADE; TABOGA, SEBASTIAO ROBERTO. Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils. Experimental and Molecular Pathology, v. 113, APR 2020. (15/01548-5, 16/22947-8)

RIVAS LEONEL, ELLEN CRISTINA; PEGORIN CAMPOS, SILVANA GISELE; ALVES GUERRA, LUIZ HENRIQUE; BEDOLO, CAROLINA MARQUES; LEITE VILAMAIOR, PATRICIA SIMONE; CALMON, MARILIA FREITAS; RAHAL, PAULA; AMORIM, CHRISTIANI ANDRADE; TABOGA, SEBASTIDO ROBERTO. Impact of perinatal bisphenol A and 17 beta estradiol exposure: Comparing hormone receptor response. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 188, JAN 30 2020. (15/01548-5, 16/22947-8)

LEONEL, ELLEN C. R.; FALLEIROS JUNIOR, LUIZ R.; CAMPOS, SILVANA G. P.; TABOGA, SEBASTIAO R.. Histological and immunohistochemical characterization of the Mongolian gerbil's mammary gland during gestation, lactation and involution. ACTA HISTOCHEMICA, v. 119, n. 3, p. 273-283, 2017. (15/01548-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LEONEL, Ellen Cristina Rivas. Effects of neonatal exposure to bisphenol A and 17-β estradiol on the mammary gland of adult female Mongolian gerbil. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.