Listeria monocytogenes research in the set of milk of family farming properties in Botucatu-SP

• Grant number: 15/11571-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2015
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Helio Langoni
• Grantee: Gabriela Capriogli Oliveira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Despite its nutritional qualities, due to its composition, milk is considered an excellent environment for the growth of pathogenic or spoilage microorganisms. Cooling is an important method of conservation of this raw material, however, if refrigerated for long periods of time, raw milk samples may lose quality and can be associated with the growth of psicotrophic bacteria. Within this group of microorganisms, including pathogenic genera, is Listeria monocytogenes. The detection of this microorganism in food products demands interest not only for their pathogenic effects on human and animal health, but by its economic importance. Thus, the aim of this study is to perform the Listeria monocytogenes research in milk sample set of 18 small family dairy farms located in the mountainous Baixada the Botucatu SP. The samples will be taken in 15-day intervals, totaling 5 samples per property. The samples will be taken after homogenization of raw milk cans of each property and the microbiological analysis will be carried out by cultivation in PALCAM agar and molecular, by Polymerase Chain Reaction (PCR) for the detection of Listeria monocytogenes. The prevalence of positive cans will be estimated in the population of tanks with confidence interval of 95% and the analysis will be performed using the SAS (2011).

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Gabriela Capriogli. Research of Listeria monocytogenes in the bovine milk of family farms. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp).