A metagenomic and predictive modeling approach to the study the microbial ecology ...
| Grant number: | 18/24540-8 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | August 01, 2019 |
| Effective date (End): | July 31, 2021 |
| Field of knowledge: | Health Sciences - Collective Health - Public Health |
| Principal Investigator: | Anderson de Souza Sant'Ana |
| Grantee: | Celso Fasura Balthazar |
| Home Institution: | Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
Abstract Sheep milk, a food whose production and consumption is growing in the country, is an excellent source of nutrients. Some farmers prefer to freeze the milk until collect enough volume for processing and production dairy sheep milk products. The pasteurization has been used for milk conservation because of the effect of heating (time x temperature) destroys spoilage agents and pathogens. However, the same heat may destroy milk nutrients such as functional compounds and substances responsible for sensorial quality of that matrix. Ohmic heating is a process that electric energy is transformed into thermal energy, aiming high quality products without degradation of functional compounds provided by conventional thermal processes, but with the same food safety claim. Listeria monocytogenes is a pathogen bacterium with high prevalence in milk and dairy, including from sheep milk, casing listeriosis and death to immunocompromised people. As listeriosis, in the last decade, there was many food outbreaks related to Escherichia coli O157:H7 from raw sheep milk used to make cheeses. Thus the present project aims: (1) compare microbial ecology of yogurt during shelf-life (28 days, 4 °C ±1), produced with fresh and frozen (30 days, -20 °C ±2) sheep milk processed by ohmic heating and conventional pasteurization, using amplicon sequencing gene 16S rRNA; and (2) determinate inactivation kinetic of pathogens (Listeria monocytogenes and Escherichia coli O157) to establish the process condition for microbiological safety of sheep milk processed by ohmic heating. (AU) | |