Effects of oral administration of the linoleic (LA) fatty acid on the wound healing process in Diabetic mice

• Grant number: 15/17522-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2015
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Hosana Gomes Rodrigues
• Grantee: Mariah Boralli Prudente dos Anjos
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 13/06810-4 - Mechanisms of action of omega-3 and omega-6 in the tissue repair process: neuro-immune focus, AP.JP

Abstract

Type 1 diabetes mellitus, a non-transmissible autoimmune disease, presents negative changes in some systems of the body of its carriers, among them is the skin (and its healing process) and in turn lower limbs, which present delay in wound closure , Exposing diabetics to infectious agents that can cause the tissue damage to have a late conclusion leading to ulcerations with subsequent amputations. Due to this complication the recovery of damaged tissue should occur as soon as possible. However, this process undergoes modulations from elements such as fatty acids. Fatty acids are precursors of eicosanoids, lipid mediators of inflammation, and therefore can modulate the healing process. Results with linoleic acid (LA), an omega-6 fatty acid, were shown to be favorable for healing, with indications of its action on the angiogenesis process. Thus, it was shown the need to investigate in detail the influence of LA on this process and how this may impact the healing of diabetic mice. In the present study, male C57black / 6 mice will be divided into three groups: (C) control; (D) diabetic animals, induced by streptozotocin (STZ); (DLA) diabetic animals that will receive LA (50 ¼L) by oral gavage for 5 days. After this period the animals will undergo surgery of induction of the wound in the dorsal region. The wounds of the mice of each group, in the times collected, will be homogenized for the determination of cytokines and growth factors by the ELISA method, as well as the evaluation of the expression of angiogenesis genes through the array-PCR technique. The data will be analyzed by ANOVA and Tukey post-test.