Molecular research of pathogenic fungi in bats from Botucatu-SP region

• Grant number: 15/09337-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2015
• End date: September 30, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Helio Langoni
• Grantee: Brunna Mayla Vasconcelos Adorno
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Currently, zoonoses represent the majority of emerging diseases and bats play an important role as a source of infection or transmission of infectious agents. Histoplasmosis, cryptococcosis and paracoccidioidomycosis are fungal diseases reported in several animal species, and its agents are isolated of caves, caverns, soil and animal feces. Thus the aim of this study is to perform molecular detection of the fungus Histoplasma capsulatum, Cryptococcus spp. and Paracoccidioidis brasiliensis in bats in Botucatu and region in the state of São Paulo. It will be collected lung, spleen and liver bats during ten months for research and diagnosis of H. capsulatum, Cryptococcus neoformans and P. brasiliensis using the molecular diagnostic technique: Polymerase Chain Reaction (PCR). It will be held the georeferencing of the samples with the aid of Arcgis 10.1® software to generate distribution maps of points of collection of animals. The study design will be case series, analyzing the frequency of the sampled species, animal habits and found location. Statistical analysis will be conducted for the distribution and frequency of traits of interest cases as: species, feeding habits of bats and found location. Then it will be subjected to analysis with PROC FREC.Keywords: fungi, bats, systemic mycoses, PCR. (AU)