Evaluation of the pulp tissue Wistar rat molars subjected to bleaching procedure and different application protocols of remineralizing MI Paste Plus: an in vivo study

• Grant number: 15/10984-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2015
• End date: September 30, 2017
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Jéssica Galbiati Barbosa
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Dental bleaching has the ability to cause damage in the enamel and dentin, and the pulp cells due to the presence of hydrogen peroxide (H2O2). Remineralizing agents having fosfopeptídio casein and amorphous calcium phosphate (CPP-ACP), as MI Paste Plus, has been used to minimize changes in hardness and roughness of enamel after bleaching procedure. However, these studies did not examine whether there beneficial effects on pulp tissue. Objective: To investigate the effects of remineralizing MI Paste Plus (CPP-ACP), on the pulp tissue of whitened Wistar rat molars. Methods: 30 rats will be divided into 6 groups of 10 hemi-jaws each, as follows: Group Control- not receive any treatment; Cla- group will receive 30' the whitening gel H2O2 35%; Cla}Rem- group receives H2O2 35%, followed by an application 30' MI Paste Plus; Group Rem}Cla- receive MI Paste Plus, followed by H2O2 35%; Rem}Cla}Rem- Group MI Paste Plus before and after H2O2 35%; Group CLA+Rem receive 30' of the a mixture of 35% H2O2 with MI Paste Plus. After 2 and 30 days, the animals will be euthanized and the jaws removed and processed for histological evaluation (HE). Analysis method: The tissue sections stained with HE will be evaluated by predetermined criteria by assigning scores to microscopic events. Statistical tests will be performed Kruskall Wallis and Dunn (p<0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, JESSICA GALBIATI; BENETTI, FRANCINE; DE OLIVEIRA GALLINARI, MARJORIE; CARMINATTI, MARINA; DA SILVA, ANNA BEATRIZ DIAS; LOPES, ISABELA NAKAHARA IKEDA; BRISO, ANDRE LUIZ FRAGA; CINTRA, LUCIANO TAVARES ANGELO. Bleaching gel mixed with MI Paste Plus reduces penetration of H2O2 and damage to pulp tissue and maintains bleaching effectiveness. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 3, p. 1299-1309, MAR 2020. (15/10984-3)