Venomics Platform: Pachycondyla villosa venom proteome and integrated analysis of omics data

• Grant number: 15/17466-8
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 11, 2016
• End date: January 10, 2017
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Eliane Candiani Arantes Braga
• Grantee: Camila Takeno Cologna
• Supervisor: Loic Quinton
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Université de Liège (ULg), Belgium
• Associated to the scholarship: 13/26200-6 - Venomics Plataform of Pachycondyla villosa Proteome, Transcriptome, Cloning and Expression of toxins with biotechnological potential, BP.PD

Abstract

Animal venoms are considered a rich source of biologically active compounds, which has been constantly selected and refined by the processes of natural evolution, in which each molecule is endowed with pharmacological properties highly valuable for scientific purposes. It is estimated that the total number of molecules present in animal venoms is a collection of 40 million different compounds and, despite the efforts made, less than 0,01% of those compounds was identified and characterized to date. Despite the commitment, the exploration of these bio-libraries remains limited which might be related to the technological limitations that prevent full-scale investigation of these venoms. In addition, the conventional methods used to explore animal venoms are still time-consuming and require large amounts of samples, which restrict the studies for a few species. However, recent advances in transcriptomic and proteomic techniques have contributed to the advance of this promising niche. The sensitivity improvement of these instruments allows the study of minimal amounts of sample still yielding a wealth of information. This project aims to perform a proteomic analysis of the venom from the ant Pachycondilyla villosa focusing on the de novo sequencing and the characterization of post translational modifications (identification and localization) using high resolution mass spectrometers. In addition, this project aims to combine this proteomics data and the transcriptomic data obtained on the first year of post doctoral fellowship, achieving therefore the full landscape of Pachycondyla villosa venom.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMILA TAKENO COLOGNA; RENATA SANTOS RODRIGUES; JEAN SANTOS; EDWIN DE PAUW; ELIANE CANDIANI ARANTES; LOÏC QUINTON. Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, 2018-03-26. (15/17466-8, 13/26200-6, 15/18432-0)

AMORIM, FERNANDA GOBBI; LONGHIM, HELOISA TAVONI; COLOGNA, CAMILA TAKENO; DEGUELDRE, MICHEL; DE PAUW, EDWIN; QUINTON, LOIC; ARANTES, ELIANE CANDIANI. Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, APR 18 2019. (14/07824-1, 15/17466-8, 12/14996-8, 13/26083-0, 11/23236-4, 14/22959-0, 13/26200-6)

WIEZEL, GISELE A.; SHIBAO, PRISCILA Y. T.; COLOGNA, CAMILA T.; MORANDI FILHO, ROMUALDO; UEIRA-VIEIRA, CARLOS; DE PAUW, EDWIN; QUINTON, LOIC; ARANTES, ELIANE C.. In-Depth Venome of the Brazilian Rattlesnake Crotalus durissus terrificus: An Integrative Approach Combining Its Venom Gland Transcriptome and Venom Proteome. JOURNAL OF PROTEOME RESEARCH, v. 17, n. 11, p. 3941-3958, NOV 2018. (15/17466-8, 13/26200-6, 11/23236-4, 17/00586-6, 14/15644-3, 15/18432-0)