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Morphological and Behavioral Changes in Ischemic Gerbils Induced by Exposure to Nicotine and Forced Continuous Treadmill Training.

Grant number: 15/07730-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): November 01, 2015
Effective date (End): September 30, 2016
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:João Eduardo de Araujo
Grantee:Takae Tamy Kitabatake
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Nicotine is a natural liquid alkaloid found in the cigarette in its particulate phase. It is considered a licit drug, freely traded worldwide and with a tendency to increased consumption, in that case it is directly or indirectly responsible for millions of deaths annually. In animal studies, nicotine has significant an complex effects on locomotor system, depending on the age and sex of the tested animal. Stroke is an important neurological disease and can cause permanent damage. Through the mechanisms of ischemic stroke, even short periods of time is capable of promoting cell death and consequent damage to brain functions. The use of animal models allows the research of the pathophysiology of brain ischemia especially global ischemia models. In this matter, physical activity is effective in maintaining the health of the individual and brain structures with beneficial effects on memory and learning. In the present work we will expose gerbils to nicotine and produce ischemia. The effects of the exposure and surgery will be analyzed in the areas M1, CA1 and striatum, as well as its behavioral impact after the forced continues gait training on a treadmill by 5 consecutive days. Will use the time 15 minutes with a speed of 5m/min. For this, 110 gerbils will be used, divided into 11 groups (n = 10) CG (Control Group), CGS (Control Group Salina), CGN (Control Group Nicotine), SG (Sham Group), SGS (Sham Group Salina) , SGN (Sham Group Nicotine), SGEN (Sham Group Stimulated Nicotine), IG (Ischemic Group), IGS (Ischemic Group Salina), IGN (Ischemia Group Nicotine) and IGEN (Ischemic Group ischemia Stimulated Nicotine). For nine consecutive days, each animal of nicotine groups will receive the nicotine intraperitoneally with an injection of 1 mg / kg (-) - nicotine (Sigma) dissolved in saline, recalculated volume with 1mg of nicotine and 1 ml saline. The animals of saline groups will be subjected to the same protocol, but with saline administration. After 3 days of application of nicotine the animals in te groups (GI, GIS, GIN GIEN) will have both carotid arteries exposed and occluded by with a suture for 5 minutes. The Sham groups (GS, GSS, GSN and GSNE) will have the procedure interrupted before occlusion. The animals of GSEN and GIEN group will be submitted to motor stimulation in 5 consecutive days, beginning 24 hours after the surgery. The stimulation protocol is 5m / min for 15 minutes. On the sixth day after surgery will be collected motor behavior data in the Activity Monitor, and the balance of animals in Rota Rod. After the behavioral testing, the animals will be anesthetized, perfused and have their brains collected for histological analysis of the hypothalamus (CA1), striatum and motor area M1.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASSIANO SILVEIRA, ANA PAULA; KITABATAKE, TAKAE TAMY; PANTALEO, VIVIAN MOZOL; ZANGROSSI JUNIOR, HELLO; BERTOLINO, GUILHERME; DE OLIVEIRA GUIRRO, ELAINE CALDEIRA; DUTRA DE SOUZA, HUGO CELSO; DE ARAUJO, JOAO EDUARDO. Continuous and not continuous 2-week treadmill training enhances the performance in the passive avoidance test in ischemic gerbils. Neuroscience Letters, v. 665, p. 170-175, . (05/02607-3, 15/07730-0, 07/03077-3)
BRITO, RAQUEL CARDOSO; OLIVATO, THAIRYNE; KITABATAKE, TAKAE TAMY; ZHANG, KELLY; GUIRRO, ELAINE CALDEIRA DE OLIVEIRA; DE ARAUJO, JOAO EDUARDO. Static magnetic field blocked alprazolam-induced behavior of Wistar rats in the elevated plus-maze test. Neuroscience Letters, v. 794, p. 6-pg., . (14/26561-1, 15/07730-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
KITABATAKE, Takae Tamy. Morphological and behavioral changes in ischemic gerbils induced by nicotine exposure and to continuous treadmill training. 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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