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MOLECULAR BIOINDICATORS OF SOIL MICROBIOME FROM CORN CROP UNDER STRAW EFFECT TO N2O GAS MITIGATION

Grant number: 15/08564-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2015
Effective date (End): May 31, 2018
Field of knowledge:Agronomical Sciences - Agronomy - Soil Science
Principal Investigator:Tsai Siu Mui
Grantee:Caio Augusto Yoshiura
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):16/11268-2 - Uncovering microbial greenhouse gas flux mediators of the maize rhizosphere: how does Azospirillum brasiliense mitigate emissions?, BE.EP.DR

Abstract

Brazil occupies a prominent place in maize production, as the third largest producer in the world. However, current papers about corn crop focus mainly on the potential production of plant and its nutrition, little exploring the role of microorganisms in the soil. Therefore, it is fundamentally important to understand the microbiological processes for soil quality maintenance and the mitigation of GHG emissions in this agricultural system with high demand for nitrogen. In this approach, the use of molecular biology allow explore potential microbial balance groups as biomarkers of biological processes. The microarray platform in the taxonomic and functional analysis (GeoChip) has generated results accurately on microorganisms in biogeochemical cycles. About this project, GHG emission fluxes and qPCR obtained data from macrocosm experiment showed straw as a potential attenuator agent of N2O flux. This way, a second experiment was designed with the purpose of elucidating the dynamics of active microbiome around this input by messenger RNA sequencing. Therefore, the development of this project is to generate information on the interaction of urea, diazotrophic inoculant and straw in rhizospheric community of maize in tropical soils; in order to identify molecular bioindicators related to mitigation of N2O. Consequently, we will apply the microarray platform (GeoChip 5.0) to determine the taxonomic and functional aspects of microbial communities, evaluating their participation in biogeochemical cycles and correlate the GHG fluxes under controlled experiment in macrocosm. Moreover, a second experiment will be developed to elucidate the processes of decomposition of organic matter and availability of straw that maintain the dynamics of microbial communities, which could be used to exploration of specific functional genes for lignocellulose decomposition of straw.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VENTURINI, ANDRESSA MONTEIRO; NAKAMURA, FERNANDA MANCINI; GONTIJO, JULIA BRANDAO; DA FRANCA, ALINE GIOVANA; YOSHIURA, CAIO AUGUSTO; MANDRO, JESSICA ADRIELE; TSAI, SIU MUI. Robust DNA protocols for tropical soils. HELIYON, v. 6, n. 5 MAY 2020. Web of Science Citations: 0.
BRAGA, LUCAS P. P.; YOSHIURA, CAIO A.; BORGES, CLOVIS D.; HORN, MARCUS A.; BROWN, GEORGE G.; DRAKE, HAROLD L.; TSAI, SIU M. Disentangling the influence of earthworms in sugarcane rhizosphere. SCIENTIFIC REPORTS, v. 6, DEC 15 2016. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.