In vitro embryos production (IVP) stands out as reproductive biotechnology in cattle herds, but still with some limitations. The difference between bulls fertility is one of them and only a few studies aimed to understand which aspects characterize this difference. The sperm cell carries besides the paternal DNA, the entire nucleus content attached to it, so it is important to understand better the composition of sperm nucleus and the changes that go beyond the sequence of bases, such as epigenetic signals. According to this, the aim of this study is to identify which sperm nucleus features characterize the difference between bulls on in vitro production rates. A data base of IVP from commercial bulls will be used to select bulls with high and low fertility. Once established these groups, it will be evaluate the chromatin integrity (SCSA modified, CMA3, aniline blue test and COMET assay), nuclear protein profile (2D-DIGE and identification of differentially expressed proteins) and epigenetic signals (global assessment DNA methylation and histone evaluation of genes related to embryonic development) between these two groups.
News published in Agência FAPESP Newsletter about the scholarship: