The pyraclostrobin is a fungicide which has been used to delay senescence and oxidative stress of many crops, contributing also to virus attack resistance. The aim of this work is to verify whether the treatment of mulberry plants with pyraclostrobin inhibits the presence of fungus and increases resistance to BmNPV in Bombyx mori caterpillars, considering the eventual bioenergetic dysfunction and the impact on mortality rates in the caterpillars. In three seasons (summer, autumn and spring), during 2015 and 2016, we will use a mulberry crop (IZ56/4 variety) treated with pyraclostrobin (0, 100, 200 and 300 g / ha of active ingredient), applied on a solution of 1,000 L / ha, after 60 and 75 days of each pruning of mulberry branches. Regarding silkworm caterpillars on fifth instar fed on mulberry leaves of plants treated with pyraclostrobin, it will be evaluated the leaf consumption, mortality of caterpillars, fungal contamination in the rearing bed, susceptibility to BmNPV and mitochondrial bioenergetics "in vivo" and "in vitro" of the head and midgut of silkworm caterpillars. The data will be submitted to analysis of variance and for multiple comparisons will be applied the 5% Tukey test, except to the mitochondrial bioenergetics data, for which will be analyzed the contrasts to evaluate the polynomial effect of the doses on the variable studied, followed by Dunnett's test.
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