Involviment of toll-like receptor type 4 (TLR4) in a hippocampal injury in mouse subject in anoxia neonatal

• Grant number: 15/19657-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2015
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Silvia Honda Takada
• Grantee: Beatriz Crossiol Vicente de Campos
• Host Institution: Centro de Matemática, Computação e Cognição (CMCC). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Neonatal anoxia is an important public health concern due to severe and permanent sequelae such as cerebral palsy, cognitive, hearing and visual deficits. Cellular responses to oxygen deprivation are quite complex and various types of processes are involved at different stages of the injury, including cell death and inflammation, especially in vulnerable regions such as the hippocampus and cerebral cortex. The Toll-like receptors (TLRs) are key components of innate and adaptive immune system that recognize a wide range of molecular patterns associated with pathogens. In this study, we propose the study of the involvement of TLR4 in hippocampal lesions of mice subjected to neonatal anoxia. For this, we will standardize a mice model of neonatal anoxia previously standardized and validated in rats, whose brain development corresponds to a human premature, given that prematurity is an important risk factor for the occurrence of neonatal anoxia. We will use mice with spontaneous mutation in the gene encoding TLR4, so in these animals there is no action of TLR4 triggering inflammatory cascades. Thus, providing data to better understand the mechanisms that lead to injury hippocampal forward to oxygen deprivation, we hope to contribute to propose new therapeutic strategies to minimize the after-effects caused by neonatal anoxia.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

IKEBARA, JULIANE MIDORI; TAKADA, SILVIA HONDA; CARDOSO, DEBORA STERZECK; MORALLES DIAS, NATALIA MYUKI; VICENTE DE CAMPOS, BEATRIZ CROSSIOL; SANCHES BRETHERICK, TALITHA AMANDA; VILAR HIGA, GUILHERME SHIGUETO; AYRES FERRAZ, MARIANA SACRINI; KIHARA, ALEXANDRE HIROAKI. Functional Role of Intracellular Calcium Receptor Inositol 1,4,5-Trisphosphate Type 1 in Rat Hippocampus after Neonatal Anoxia. PLoS One, v. 12, n. 1, JAN 10 2017. (14/15018-5, 14/16711-6, 15/17044-6, 15/19699-0, 15/19657-5, 14/17434-6)