EFFECT OF PALM JUÇARA FRUIT (Euterpe edulis Mart.) SUPPLEMENTATION ON EPIGENETIC MODIFICATIONS AND INFLAMMATORY STATUS IN MONOCYTES OF OBESE ADULTS.

• Grant number: 15/13875-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): November 01, 2015
• Effective date (End): June 30, 2020
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Luciana Pellegrini Pisani
• Grantee: Aline Boveto Santamarina
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated scholarship(s): 18/23623-7 - The effects of oleic acid and cyanidin supplementation of macrophages on the modulation of inflammatory processes, BE.EP.DR

Abstract

Introduction: Currently obesity is established as a public health problem and requires interventions that allow combating them. Installation of chronic subclinical inflammation and epigenetic alterations are related to pathology of this multifactorial disease. Thus the search for foods able to combat and prevent these diseases became necessary. In this sense, the fruit of juçara palm (Euterpe edulis Mart.) seems to be a food with potential against obesity due to its composition rich in anthocyanins, polyunsaturated fatty acids and dietary fiber. Objective: To analyze the effect of the pulp of the fruit of Juçara Palm consumption, on the pro-inflammatory state of obesity and epigenetic modifications. Methods: 72 subjects with obesity (BMI 30.0 to 39.9 kg / m2) of both genders aged 31 to 59 will be randomized divided into 4 groups in use or not (placebo) of fruit pulp supplementation during 8 weeks. Will be held anthropometry, assessment of food intake, profile of fatty acids in serum by gas chromatography, epigenetic markers (global DNA methylation in monocytes; protein and gene expression of proteins involved in epigenetic mechanisms: DNMT1, DNMT3a , DNMT3B and MeCP2, and HDAC enzyme activity in monocytes); The monocyte inflammatory profile of TLR4 / NFºB activation cascade by gene expression of the following proteins associated with inflammation: TLR4, TLR2, IL-6R, IL-10R±, TNFR1, AdipoR2, IL-10, IL-6, TNF, -±, Ob-R; and protein expression of the following proteins: TLR4, TLR2, IL-6R, IL-10R±, TNFR1, AdipoR2, Ob-R, MyD88, TRAF6, and all fractions and phosphorylated IKK ± / ² and NF-kB (p50 subunits and p65).

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTAMARINA, ALINE BOVETO; JAMAR, GIOVANA; MENNITTI, LAIS VALES; CESAR, HELENA DE CASSIA; DE ROSSO, VERDIANA VERA; VASCONCELOS, JOSE RONNIE; OYAMA, LILA MISSAE; PISANI, LUCIANA PELLEGRINI. Supplementation of Jucara Berry (Euterpe edulis Mart.) Modulates Epigenetic Markers in Monocytes from Obese Adults: A Double-Blind Randomized Trial. NUTRIENTS, v. 10, n. 12, DEC 2018. (15/13875-0, 16/14133-0)

SANTAMARINA, ALINE B.; PISANI, LUCIANA P.; BAKER, ELLA J.; MARAT, ANDREEA D.; VALENZUELA, CARINA A.; MILES, ELIZABETH A.; CALDER, PHILIP C.. Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway. FOOD & FUNCTION, v. 12, n. 17, JUN 2021. (18/23623-7, 15/13875-0)

SANTAMARINA, ALINE BOVETO; JAMAR, GIOVANA; MENNITTI, LAIS VALES; RIBEIRO, DANIEL ARAKI; CARDOSO, CAROLINE MARGONATO; DE ROSSO, VERIDIANA VERA; OYAMA, LILA MISSAE; PISANI, LUCIANA PELLEGRINI. Polyphenols-Rich Fruit (Euterpe edulis Mart.) Prevents Peripheral Inflammatory Pathway Activation by the Short-Term High-Fat Diet. Molecules, v. 24, n. 9, MAY 1 2019. (18/23623-7, 15/13875-0, 16/14133-0)

PISANI, LUCIANA PELLEGRINI. The Use of Jucara (Euterpe edulis Mart.) Supplementation for Suppression of NF-B Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats. Molecules, v. 23, n. 7, JUL 2018. (16/14133-0, 15/13875-0)

SANTAMARINA, ALINE BOVETO; JAMAR, GIOVANA; MENNITTI, LAIS VALES; CESAR, HELENA DE CASSIA; VASCONCELOS, JOSE RONNIE; OYAMA, LILA MISSAE; DE ROSSO, VERIDIANA VERA; PISANI, LUCIANA PELLEGRINI. Obesity-related inflammatory modulation by jucara berry (Euterpe edulis Mart.) supplementation in Brazilian adults: a double-blind randomized controlled trial. EUROPEAN JOURNAL OF NUTRITION, v. 59, n. 4, p. 1693-1705, JUN 2020. (15/13875-0)