To investigate the effect of desensitizing toothpastes on initial enamel erosion and abrasion, 160 enamel samples from human third molars will be prepared. They will be randomly divided into 8 groups (n=20): (G1) deionized water; (G2) Colgate Cavity Protection; (G3) Regenerate; (G4) Colgate Pro Relief; (G5) Colgate Sensitive Original; (G6) Sensodyne Repair and Protection; (G7) Sensodyne Rapid Relief; (G8) Oral-B Sensi Relief. The specimens will be submitted to an erosion-abrasion cycling model of 8 days (1 cycle/day), which consists of incubation in artificial saliva (30 min), immersion in toothpaste slurry for 2 min, with toothbrush abrasion being performed for 10 s (20 toothbrush strokes), and erosion for 2 min, in 1% citric acid (pH = 3.6). Surface microhardness (SMH) and specular reflection will be measured at the beginning of the experiment. SMH will be remeasured after each abrasion and erosion treatment, and specular reflection will be remeasured at the end of each cycle. Enamel substance loss will be calculated after each abrasion. Statistical analyses will be performed considering a significant level of 5%.
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