Effect of low-intensity laser on gene and protein expression of different phenotypes of macrophages

• Grant number: 15/21219-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2015
• End date: December 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Kristianne Porta Santos Fernandes
• Grantee: Kaline de Brito Sousa
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

The interactions between tissue and macrophages that invade after an injury happens are crucial to the evolution of its repair. On the other hand, low intensity laser (LLLT) has been extensively used in the treatment of tissue damage in different clinical and experimental contexts; however, little is known about its effects on macrophages. The objectives of this project are to evaluate the effects of LLLT (2 dosimetric parameters) on gene expression and protein synthesis of 12 products (important in tissue repair) of activated/polarized macrophages (for phenotypes M1, M2a and M2C). Macrophage cultures will be activated/polarized for 24 hours, washed and irradiated with red laser (660 nm) and infrared (780 nm) in the same dosimetric parameters (70 mW, 17,5 J/cm2, 0,8J). Following incubation periods of 4 and 24 hours, PCR array analysis will be performed for gene expression of chemokines (cxcl2 (MIP-2a), ccl 2 (Mcp-1), ccl 3 (Mip-1a), ccl4 (Mip-1b)); cytokines (IL-1², IFN³, IL-6, TNF±, IL-13); and growth factors (TGF²1, IGF1 and Vegfa). Culture supernatant of the different experimental groups (after 24 hours of irradiation) will be used to assess protein production. In each experimental situation, non-irradiated and non-activated cells will serve as control. Three independent experiments will be conducted within each aforementioned methodology. All results will be analyzed statistically.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUSA, KALINE DE BRITO; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; SANTOS, DEBORA DE SOUZA; MESQUITA-FERRARI, RAQUEL AGNELLI; NUNES, FABIO DAUMAS; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA KALIL; SANTOS FERNANDES, KRISTIANNE PORTA. Differential expression of inflammatory and anti-inflammatory mediators by M1 and M2 macrophages after photobiomodulation with red or infrared lasers. Lasers in Medical Science, v. 35, n. 2, p. 337-343, MAR 2020. (15/21219-6)

DE BRITO SOUSA, KALINE; DE FATIMA TEIXEIRA DA SILVA, DANIELA; RODRIGUES, MARIA FERNANDA SETUBAL DESTRO; GARCIA, MONICA PEREIRA; DE OLIVEIRA RODINI, CAROLINA; MESQUITA-FERRARI, RAQUEL AGNELLI; HAMBLIN, MICHAEL R.; BUSSADORI, SANDRA KALIL; NUNES, FABIO DAUMAS; FERNANDES, KRISTIANNE PORTA SANTOS. Effects of the phenotypic polarization state of human leukocytes on the optical absorbance spectrum. Journal of Biophotonics, v. 14, n. 6, MAR 2021. (15/21219-6, 13/07502-1)