Unraveling the effects of UCP1 overexpression during reproduction and upon stability of Group VII ethylene response factor transcription factors

• Grant number: 15/24881-1
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 01, 2016
• End date: July 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Paulo Arruda
• Grantee: Pedro Paulo Augusto Fabiano Arantes Pereira Barreto
• Supervisor: Zoe Wilson
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: University of Nottingham, Sutton Bonington, England
• Associated to the scholarship: 14/17634-5 - Mitochondria as a key element on stress response in plants, BP.PD

Abstract

Mitochondria are the reason eukaryotic cells could arise more than a billion years ago. It is the most ancient and interconnected organelle inside the eukaryote cell, which obligate them to preserve the organelle integrity to stay alive. The mitochondrial uncoupling proteins (UCPs) are among the ~1,000 proteins that compose the mitochondrial architecture and play a key role in securing integrity under stress. We have demonstrated that the constitutive overexpression of UCP1 induces a broad metabolic reconfiguration in the cellular context and makes plants more tolerant to several biotic and abiotic stresses. Despite the observation that UCP1 overexpressors perform better under stress, the impact of this on flower development, an organ that plays key role in plant seed production, is still unknown. Flower formation and in particular pollen development, has a high demand for energy, which is manifest as a major increase in mitochondria number and activity. Defects in mitochondria function result in male sterility, emphasising the importance of the mitochondria during this stage of development. Flowering is also extremely vulnerable to abiotic stress, resulting in sterility or reduced seed set. This project aims to explore the effect of UCP1 and other mitochondrial genes overexpression in Arabidopsis during growth and in response to stressful environments, focusing on oxygen-conditional stability of Group VII Ethylene Response Factor transcription factors, as we recently demonstrated that UCP1 overexpression induces a hypoxic response in tobacco leaves.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARRETO, PEDRO; DAMBIRE, CHARLENE; SHARMA, GUNJAN; VICENTE, JORGE; OSBORNE, RORY; YASSITEPE, JULIANA; GIBBS, DANIEL J.; MAIA, IVAN G.; HOLDSWORTH, MICHAEL J.; ARUDA, PAULO. Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway. Current Biology, v. 32, n. 6, p. 14-pg., 2022-03-28. (15/24881-1, 14/17634-5, 16/23218-0, 17/22745-9)