Study of photosensitized radical-mediated lipid oxidation by the use of a BODIPY-a-tocopherol off/on fluorescent probe in model membranes

• Grant number: 15/22935-7
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Effective date (Start): May 01, 2016
• Effective date (End): October 31, 2016
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Mauricio da Silva Baptista
• Grantee: Isabel de Oliveira Lima Bacellar
• Supervisor: Gonzalo Cosa
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Research place: McGill University, Montreal, Canada
• Associated to the scholarship: 13/11640-0 - Relationship between the photoinduced damage in lipids and membrane permeabilization, BP.DD

Abstract

Radical-mediated oxidations are expected to play an important role on photosensitized oxidations occuring in photodynamic therapy and cutaneous photoaging. Given the role of lipid photo-oxidation in these photobiological processes, in this project we aim to study the formation of lipid peroxyl radicals in model membranes, by using of BODIPY-a-tocopherol off/on fluorescent probes. These probes, which were developed by the group of Prof. Dr. Gonzalo Cosa at McGill University, show enhanced fluorescence upon reaction with peroxyl radicals. For that reason, they allow tracking the onset of lipid oxidation both by bulk measurements and fluorescence imaging techniques. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAKAYA, AYA; BACELLAR, ISABEL O. L.; FONSECA, JOSE LUIS; DURANTINI, ANDRES M.; MCCAIN, JULIA; XU, LAIYI; VIGNONI, MARIANA; THOMAS, ANDRES H.; BAPTISTA, MAURICIO S.; COSA, GONZALO. Singlet Oxygen Flux, Associated Lipid Photooxidation, and Membrane Expansion Dynamics Visualized on Giant Unilamellar Vesicles. Langmuir, v. N/A, p. 11-pg., 2022-12-28. (13/11640-0, 13/07937-8, 15/22935-7)

BACELLAR, ISABEL O. L.; OLIYEIRA, MARIA CECILIA; DANTAS, LUCAS S.; COSTA, ELIERGE B.; JUNQUEIRA, HELENA C.; MARTINS, WALESKA K.; DURANTINI, ANDRES M.; COSA, GONZALO; DI MASCIO, PAOLO; WAINWRIGHT, MARK; et al. Photosensitized Membrane Permeabilization Requires Contact-Dependent Reactions between Photosensitizer and Lipids. Journal of the American Chemical Society, v. 140, n. 30, p. 9606-9615, AUG 1 2018. (13/11640-0, 15/22935-7, 13/07937-8, 12/50680-5)