Influence of biosilicate based experimental gel in the inflammatory process of the pulp tissue due to dental bleaching

• Grant number: 15/22996-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2016
• End date: December 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Marina Carminatti
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

It is known that hydrogen peroxide (H2O2), the active substance of the dental bleaching gel, can penetrate through the enamel and dentin producing changes in these tissues and cause damage to pulp tissue. Some formulations based on Biosilicate® (BS) have been shown to minimize induced changes in enamel and dentin following dental bleaching. It was also observed positive effect on remineralization of dental tissues, minimizing dentin hypersensitivity. Considering the possibility of developing new products that can minimize the effects of H2O2 on the pulp tissue, the aim of this study is: - to evaluate the therapeutic potential of an BS based experimental gel on the pulp tissue of bleached Wistar rat molars function of different application protocols. Methods: 80 hemimaxilae of 40 rats will be randomly divided into 8 groups of 10 hemimaxilae each: Control group- not receive any treatment; Cla group- will receive 30 minutes of bleaching gel 35% H2O2; Cla}BS/20min group- receive 35% H2O2, followed by application of 20 min BS gel; Cla}BS/14d group- receive 35% H2O2, and an application of 30 sec BS gel every 72 hours for 14 days; BS/20min}Cla group- receive 20 min BS gel, followed by 35% H2O2; BS/7d}Cla group- receive an application 20 min BS gel every 24 hours for 7 days, and on day 7, 35% H2O2; Cla+BS group- receive 30 minutes of a mixture of 35% H2O2 to BS; BS/7d group- receive an application 20 min BS every 24 hours for 7 days. After 2 and 30 days, the animals will be euthanized. Analysis method: the hemimaxilae will be processed for histological evaluation (HE). Scores are assigned to the inflammatory infiltrate, and applied the Kruskal-Wallis test and Dunn (p<.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARINA CARMINATTI; FRANCINE BENETTI; RENATO LUIZ SIQUEIRA; EDGAR DUTRA ZANOTTO; ANDRÉ LUIZ FRAGA BRISO; ANTÔNIO HERNANDES CHAVES-NETO; LUCIANO TAVARES ANGELO CINTRA. Experimental gel containing bioactive glass-ceramic to minimize the pulp damage caused by dental bleaching in rats. Journal of Applied Oral Science, v. 28, 2020-06-08. (13/07793-6, 15/22996-6)