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EFFECT OF LOW LEVEL LASER THERAPY AND EPIDERMAL GROWTH FACTOR ON IN VITRO BIOLOGICAL SEALING INDUCTION

Grant number: 15/19364-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2015
Effective date (End): October 31, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Carlos Alberto de Souza Costa
Grantee:Taisa Nogueira Pansani
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):18/05258-0 - Development, characterization and biological effects of a titanium surface covered with growth factor, BE.EP.DR

Abstract

The attachment of connective tissue to the surface of abutments of dental implants prevents the apical migration of the junctional epithelium and the consequent bone crest reabsorption. When bacterial contamination and peri-implant inflammation take place, cells lose their potential to adhere to the substrate, resulting in apical migration of the epithelium what compromises this aesthetic treatment. The use of therapies to accelerate or enhance cell adhesion, inducing an effective biological sealing may improve the aesthetic and functional conditions in prosthetic rehabilitation. Therefore, the aim of this study is to assess the effects of low level laser therapy (LLLT) and the impregnation of titanium (Ti) and zirconia (ZrO2) surfaces with epidermal growth factor (EGF) on adhesion and metabolism of human oral mucosa cells exposed or not to lipopolysaccharide (LPS) of Porphyromonas gingivalis (P. gingivalis) or tumor necrosis factor ± (TNF-±). For this purpose, gingival fibroblasts and epithelial cells will be seeded on Ti and ZrO2 discs, simulating an in vitro biological sealing. In groups with EGF, the growth factor will be applied to the surfaces of both materials before cell seeding. Then, the cells will be irradiated for 3 times at 24-h intervals using doses of 0.5 J/cm2; 1.5 J/cm2 and 3.0 J/cm2 according to the established groups and TNF-± or P. gingivalis LPS will be applied for 24 h. Cell viability (MTT assay), collagen quantification (Sirius Red), gene expression of VEGF, IL-6, IL-8 and TNF-± (qPCR), synthesis of VEGF, IL-6, IL-8 and TNF-± (ELISA) as well as expression of e-cadherin and phalloidin (immunofluorescence) and cell morphology analysis (MEV) will be assessed. Specific statistical testes will be selected and then used to analyze the obtained data at 5% significance level.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PANSANI, TAISA NOGUEIRA; BASSO, FERNANDA GONCALVES; DE SOUZA COSTA, CARLOS ALBERTO. In vitro effects of photobiomodulation applied to gingival fibroblasts cultured on titanium and zirconia surfaces and exposed to LPS fromEscherichia coli. Lasers in Medical Science, v. 35, n. 9 JUN 2020. Web of Science Citations: 0.
PANSANI, TAISA NOGUEIRA; BASSO, FERNANDA GONCALVES; SOUZA, ISABELA DOS REIS; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts. ARCHIVES OF ORAL BIOLOGY, v. 102, p. 48-54, JUN 2019. Web of Science Citations: 1.
PANSANI, TAISA NOGUEIRA; BASSO, FERNANDA GONCALVES; SOARES, DIANA GABRIELA; DA SILVEIRA TURRIONI, ANA PAULA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Photobiomodulation in the Metabolism of Lipopolysaccharides-exposed Epithelial Cells and Gingival Fibroblasts. Photochemistry and Photobiology, v. 94, n. 3, p. 598-603, MAY-JUN 2018. Web of Science Citations: 0.
TAISA NOGUEIRA PANSANI; FERNANDA GONÇALVES BASSO; DIANA GABRIELA SOARES; JOSIMERI HEBLING; CARLOS ALBERTO DE SOUZA COSTA. Functional Differences In Gingival Fibroblasts Obtained from Young and Elderly Individuals. Brazilian Dental Journal, v. 27, n. 5, p. 485-491, Out. 2016.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PANSANI, Taisa Nogueira. Efeito da laserterapia de baixa potência e do fator de crescimento epidérmico sobre a indução do metabolismo celular em superfícies de titânio e zircônia. 2019. 101 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Odontologia (Campus de Araraquara)..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.