The attachment of the oral mucosa to the surface of abutments of dental implants prevents undesired apical migration of the junctional epithelium and reabsorption of the bone crest. The surface modification of implants and their prosthetic components (abutments) can favor cell adhesion improving the aesthetic and functional conditions of the prosthetic rehabilitation. The objective of this study will be the development, characterization and determination of the biological effects of a bioactive titanium surface impregnated with epidermal growth factor. Titanium discs (Ti) of 8-mm diameter will be subjected to a topography modification by chemical and thermal treatment and then covered with epidermal growth factor (EGF - 100 nM). Physical characterization will be determined by Atomic Force Microscopy (AFM) and Raman Spectroscopy (Raman). To assess biological effects of this modification, a primary cultures of gingival fibroblasts and oral keratinocytes will be established and the cells will seeded on Ti discs in complete culture medium (DMEM), supplemented with 10% of fetal bovine serum (FBS) and antibiotic/antimycotic solution. After 24h-incubation, cell viability (alamarBlue assay) and cell adhesion (Confocal Microscopy) will be evaluated. To analyze the bacterial adhesion potential, a biofilm will be created with Porphyromonas gingivalis (ATCC 33277) onto the titanium discs and then subjected to viability test (XTT) and Confocal Microscopy (Live and Dead). For quantitative analysis, a significance level of 5% will be assigned.
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