Implementation of CLIP-seq computational analyzes for determination of target mRNAs and Caprin-1 protein binding sites

• Grant number: 15/25134-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2016
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Katlin Brauer Massirer
• Grantee: Felipe Eduardo Ciamponi
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 12/00195-3 - Post-transcriptional regulatory networks mediated by RNA binding proteins in human pluripotent stem cells, AP.JP

Abstract

RNA-binding proteins regulate post-transcriptionally a large number of genes. It binds to various cellular mRNAs regulating splicing, location, stabilization and degradation of transcripts and act as guides in gene regulation by short non-coding RNAs, such as microRNAs. Mutations or alterations in RBPs or alterations in target mRNA regulations are associated with diseases, failures in maintaining stem cell pluripotency and processes involved in cell differentiation. However, the action mechanisms and mRNA targets of some RBPs remains unclear. Thus, this study aims to identify the target mRNAs linked to Caprin-1 protein (or Rng-105) in addition to defining the binding sites of the protein mRNAs.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WU, QIN; NIE, DAVID Y.; BA-ALAWI, WAIL; JI, YISHUAI; ZHANG, ZIWEN; CRUICKSHANK, JENNIFER; HAIGHT, JILLIAN; CIAMPONI, FELIPE E.; CHEN, JOCELYN; DUAN, SHILI; et al. PRMT inhibition induces a viral mimicry response in triple-negative breast cancer. Nature Chemical Biology, v. N/A, p. 22-pg., 2022-05-16. (15/25134-5, 20/02006-0, 16/25521-1, 14/50897-0)

SACHAMITR, PATTY; HO, JOLENE C.; CIAMPONI, FELIPE E.; BA-ALAWI, WAIL; COUTINHO, FIONA J.; GUILHAMON, PAUL; KUSHIDA, MICHELLE M.; CAVALLI, FLORENCE M. G.; LEE, LILIAN; RASTEGAR, NAGHMEH; et al. PRMT5 inhibition disrupts splicing and stemness in glioblastoma. NATURE COMMUNICATIONS, v. 12, n. 1, FEB 12 2021. (16/25521-1, 15/25134-5, 14/50897-0, 14/21704-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CIAMPONI, Felipe Eduardo. Identificação de características biológicas associadas a sítios-alvo de proteínas de ligação ao RNA (RBPs). 2018. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.