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Implementation of CLIP-seq computational analyzes for determination of target mRNAs and Caprin-1 protein binding sites

Grant number: 15/25134-5
Support type:Scholarships in Brazil - Master
Effective date (Start): February 01, 2016
Effective date (End): February 28, 2017
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal Investigator:Katlin Brauer Massirer
Grantee:Felipe Eduardo Ciamponi
Home Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:12/00195-3 - Post-transcriptional regulatory networks mediated by RNA binding proteins in human pluripotent stem cells, AP.JP

Abstract

RNA-binding proteins regulate post-transcriptionally a large number of genes. It binds to various cellular mRNAs regulating splicing, location, stabilization and degradation of transcripts and act as guides in gene regulation by short non-coding RNAs, such as microRNAs. Mutations or alterations in RBPs or alterations in target mRNA regulations are associated with diseases, failures in maintaining stem cell pluripotency and processes involved in cell differentiation. However, the action mechanisms and mRNA targets of some RBPs remains unclear. Thus, this study aims to identify the target mRNAs linked to Caprin-1 protein (or Rng-105) in addition to defining the binding sites of the protein mRNAs.