NanoparticlesProteins interaction study based on surface functionalization of nanometric structures

• Grant number: 15/23966-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2016
• End date: December 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Mateus Borba Cardoso
• Grantee: Larissa Fernanda Ferreira
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Campinas , SP, Brazil

Abstract

The purpose of this project is to study the protein corona formation over silica nanoparticles (NPSiO2)functionalized with different groups such as thiol, amine, isocyanate and phenylethyl using model proteins(human serum albumin, human fibrinogen and human ³globulins).The impact of the surface properties on theproteinnanoparticleinteractions will be the main focus over the whole project. The nanoparticles will be fullycharacterized by small angle Xrayscattering (SAXS), transmission electron microscopy (TEM), dynamic lightscattering (DLS), zeta potential and thermogravimetric analysis (TGA). The interaction between sílicananoparticles and selected proteins will be addressed by fluorescence quenching experiments, synchronousfluorescence and fluorescence anisotropy. In addition, the nanoparticleproteincomplexes will be characterizedusing the same techniques mentioned above. It should be noted that this proposal is connected to a FAPESPproject coordinated by the proponent advisor (FAPESP 223222/2014), and also to a FAPESP postdoctoralfellowship granted to Dr. Agustin Picco (FAPESP 219108/2014) who works under the supervision of the sameproponent advisor. The granting of this proposal will positively contribute to the academic formation of thestudent Larissa Fernanda Ferreira. Moreover, this project is expected to produce relevant research results in thegrowing field of proteinnanoparticleinteractions.